Down-regulation of tissue factor by siRNA increased doxorubicin-induced apoptosis in human neuroblastoma.

Down-regulation of tissue factor by siRNA increased doxorubicin-induced apoptosis in human neuroblastoma. Research Abstract Details 

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Down-regulation of tissue factor by siRNA increased doxorubicin-induced apoptosis in human neuroblastoma. Abstract Text:

Jun Fang,Hao Tang,Linghui Xia,Muxiang Zhou,Yan Chen,Wenning Wei,Yu Hu,Shanjun Song,Mei Hong,

The effects of tissue factor (TF) on doxorubicin-induced apoptosis in human neuroblastoma were investigated. The expression of TF was examined by Western blotting. TFsiRNA-pSUPER plasmid was constructed by inserting specific 19-nt silencing sequence targeting TF gene into pSUPER vector. Transfection of TFsiRNA-pSUPER was performed using lipofectamine(2000). The cytotoxicity of doxorubicin was determined by WST assay. The activation of Caspase-3 and PARP induced by doxorubicin was tested by Western blotting. The apoptotic cells were stained by Hochest33342 and counted under fluorescence inverted microscope. It was found that human neuroblastoma cell line SK-N-MC expressed high level of TF. Knockdown of the TF expression was achieved by transfection of TFsiRNA-pSUPER on SK-N-MC cells in a dose-dependent manner. Inhibition of TF significantly decreased the viability of transfected SK-N-MC cells treated with different concentrations of doxorubicin. Cleavage of Caspase-3 and PARP was enhanced in transfected SK-N-MC cells with down-regulation of TF. TFsiRNA treatment significantly increased the number of apoptotic cells in transfected SK-N-MC cells as compared with those control cells (P<0.05) when these cells were exposed to 1 mug/mL doxorubicin for 8 h. These results suggested that knockdown of the TF expression by specific siRNA vector could increase the cytotoxicity of doxorubicin and enhance doxorubicin-induced apoptosis in human neuroblastoma cells. Over-expression of TF might contribute to chemotherapy resistance in human neuroblastoma and its progression, at lest in part, by regulating doxorubicin-induced apoptosis.

Down-regulation of tissue factor by siRNA increased doxorubicin-induced apoptosis in human neuroblastoma. Publishing Authors By Initials

J Fang,H Tang,L Xia,M Zhou,Y Chen,W Wei,Y Hu,S Song,M Hong,

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Down-regulation of tissue factor by siRNA increased doxorubicin-induced apoptosis in human neuroblastoma. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of Huazhong University of Science and Tech

VOLUME: 28

Page Numbers: 42-5

Journal Abbreviation: J. Huazhong Univ. Sci. Technol

ISSN: 1672-0733

DAY: 18

MONTH: Feb

YEAR: 2008

Down-regulation of tissue factor by siRNA increased doxorubicin-induced apoptosis in human neuroblastoma. Information

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LANGUAGE: eng

NlmUniqueID: 101169627

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AFFILIATION: Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Molecular Targeting Laboratory of Hubei Province, Wuhan, 430022, China, fangjunth@126.com.

Country: China

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MEDLINETA: J Huazhong Univ Sci Technolog

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