Dose-response relationships in gene expression profiles in rainbow trout, Oncorhyncus mykiss, exposed to ethynylestradiol.

Dose-response relationships in gene expression profiles in rainbow trout, Oncorhyncus mykiss, exposed to ethynylestradiol. Research Abstract Details 

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Dose-response relationships in gene expression profiles in rainbow trout, Oncorhyncus mykiss, exposed to ethynylestradiol. Abstract Text:

Sharon E Hook,Ann D Skillman,Jack A Small,Irvin R Schultz,Sharon E Hook,Ann D Skillman,Jack A Small,Irvin R Schultz,

Determining how gene expression profiles change with toxicant dose will improve the utility of arrays in identifying biomarkers and modes of toxic action. Isogenic rainbow trout, Oncorhyncus mykiss,were exposed to 10, 50 or 100 ng/L ethynylestradiol (a xeno-estrogen) for 7 days. Following exposure hepatic RNA was extracted. Fluorescently labeled cDNA were generated and hybridized against a commercially available Atlantic Salmon/Trout array (GRASP project, University of Victoria) spotted with 16,000 cDNAs. Transcript expression in treated vs control fish was analyzed via Genespring (Silicon Genetics) to identify genes with altered expression, as well as to determine gene clustering patterns that can be used as "expression signatures". Array results were confirmed via qRT PCR. Our analysis indicates that gene expression profiles varied somewhat with dose. Established biomarkers of exposure to estrogenic chemicals, such as vitellogenin, vitelline envelope proteins, and the estrogen receptor alpha, were induced at every dose. Other genes were dose specific, suggesting that different doses induce distinct physiological responses. These findings demonstrate that cDNA microarrays could be used to identify both toxicant class and relative dose.

Dose-response relationships in gene expression profiles in rainbow trout, Oncorhyncus mykiss, exposed to ethynylestradiol. Publishing Authors By Initials

SE Hook,AD Skillman,JA Small,IR Schultz,SE Hook,AD Skillman,JA Small,IR Schultz,

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Dose-response relationships in gene expression profiles in rainbow trout, Oncorhyncus mykiss, exposed to ethynylestradiol. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Marine environmental research

VOLUME: 62 Suppl

Page Numbers: S151-5

Journal Abbreviation: Mar. Environ. Res.

ISSN: 0141-1136

DAY: 18

MONTH: 04

YEAR: 2006

Dose-response relationships in gene expression profiles in rainbow trout, Oncorhyncus mykiss, exposed to ethynylestradiol. Information

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LANGUAGE: eng

NlmUniqueID: 9882895

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Grant and Affiliation Information for Dose-response relationships in gene expression profiles in rainbow trout, Oncorhyncus mykiss, exposed to ethynylestradiol.

AFFILIATION: Battelle Pacific Northwest Division, Sequim, WA 98382, USA. Sharon.Hook@pnl.gov

Country: England

AGENCY: United States NIEHS

GRANT: 5R01ES012446-03

ACRONYM: ES

MEDLINETA: Mar Environ Res

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