Dopamine stimulates Cl(-) absorption coupled with HCO(3)(-) secretion in rat late distal colon.

Dopamine stimulates Cl(-) absorption coupled with HCO(3)(-) secretion in rat late distal colon. Research Abstract Details 

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Dopamine stimulates Cl(-) absorption coupled with HCO(3)(-) secretion in rat late distal colon. Abstract Text:

Gui Hong Zhang,Jin Xia Zhu,Hong Xue,Jing Fan,Xin Chen,Lai Ling Tsang,Yiu Wa Chung,Ying Xing,Hsiao Chang Chan,

Freshly isolated rat colonic mucosa close to anus (the late distal colonic mucosa) was used to investigate the effect of dopamine on the rat late distal colonic ion transport by means of short-circuit current (I(SC)) recording and reverse transcription PCR (RT-PCR) analysis. The results showed that the basolateral addition of dopamine (0.1-1000 micromol/l) produced a concentration-dependent downward deflection in I(SC) with an apparent EC(50) of 20.06 micromol/l in the late distal colon. The dopamine-induced I(SC) decrease was abolished by bilateral removal of Cl(-) or HCO(3)(-), apical Cl(-) replacement and apical pretreatment with non-specific Cl(-) channel blocker/transporter inhibitor, DPC (1 mmol/l) or glibenclamide (1 mmol/l), and reversed by subsequent addition of glibenclamide. Removal of basolateral Na(+) or reducing basolateral HCO(3)(-) (3 mmol/l) as well as basolateral pretreatment with DIDS (4,4'-didsothio- cyanostilbene-2, 2'-disulfonic acid) (250 micromol/l), an inhibitor of NBC or AE, could also inhibit the dopamine-induced I(SC) response. However, apical pretreatment with epithelial Na(+) channel blocker, amiloride (10 micromol/l), Ca(2+)-dependent Cl(-) channel blocker/anion exchanger, DIDS (100 micromol/l), or putative K(+) blockers such as Ba(2+) (5 mmol/l), TEA (tetraethylammonium) (5 mmol/l) or 293B (trans-6-cyano-4- (N-ethylsulfonyl-N-methylamino)-3-hydroxy-2,2-dimethyl-chromane) (10 micromol/l) did not significantly affect the dopamine-induced I(SC) response. RT-PCR results showed the expression of anion exchanger, SLC26A3, but not SLC26A6, in rat late distal colon. In conclusion, the present results suggest that dopamine may promote rat late distal colonic epithelial Cl(-) absorption coupled with HCO(3)(-) secretion, which may be mediated by apical electrogenic anion exchanger, SLC26A3, and require basolateral entry of HCO(3)(-) through Na(+)-HCO(3)(-) cotransporter. The present findings reveal a previously unreported dopamine-regulated anion transport process in rat late distal colon, which may have implication in Parkinson's disease.

Dopamine stimulates Cl(-) absorption coupled with HCO(3)(-) secretion in rat late distal colon. Publishing Authors By Initials

GH Zhang,JX Zhu,H Xue,J Fan,X Chen,LL Tsang,YW Chung,Y Xing,HC Chan,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research

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Dopamine stimulates Cl(-) absorption coupled with HCO(3)(-) secretion in rat late distal colon. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: European journal of pharmacology

VOLUME: 570

Page Numbers: 188-95

Journal Abbreviation: Eur. J. Pharmacol.

ISSN: 0014-2999

DAY: 5

MONTH: 06

YEAR: 2007

Dopamine stimulates Cl(-) absorption coupled with HCO(3)(-) secretion in rat late distal colon. Information

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LANGUAGE: eng

NlmUniqueID: 1254354

Dopamine stimulates Cl(-) absorption coupled with HCO(3)(-) secretion in rat late distal colon. Keywords Mesh Terms:

KEYWORDS: Rats, Sprague-Dawley

MESH TERMS: metabolism

Chemical & Substance for Abstract: Dopamine stimulates Cl(-) absorption coupled with HCO(3)(-) secretion in rat late distal colon. Information

Substance Name: Dopamine

Registry Number: 51-61-6

Grant and Affiliation Information for Dopamine stimulates Cl(-) absorption coupled with HCO(3)(-) secretion in rat late distal colon.

AFFILIATION: Epithelial Cell Biology Research Center, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, PR China.

Country: Netherlands

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MEDLINETA: Eur J Pharmacol

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