Dominant role of the second heat shock element in expression of the HSP70-1 gene in rat liver after whole body hyperthermia.

Dominant role of the second heat shock element in expression of the HSP70-1 gene in rat liver after whole body hyperthermia. Research Abstract Details 

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Dominant role of the second heat shock element in expression of the HSP70-1 gene in rat liver after whole body hyperthermia. Abstract Text:

T Konishi,M Nomoto,K Shimizu,T Abe,H Itoh,

We investigated the regulatory expression of the HSP70-1 gene in rat liver after whole body hyperthermia (rectal temperature of 42 degrees C for 30 min). After heat treatment, HSP70-1 mRNA showed a peak at 1 h, and its protein product began to increase at 3 h. Under these conditions, we identified cis-acting elements involved in the regulatory expression of the HSP70-1 gene by in vivo genomic footprinting. We observed that the second heat shock element (HSE-2; -201 to -178) was clearly protected from methylation by dimethyl sulfate in parallel with the increase in HSP70-1 mRNA. On the contrary, the most proximal HSE-1 (-120 to -101) was protected only slightly. Furthermore, the flanking regions of HSE-2, that is, an NF-kappa B-like element on the upstream side and a GC box on the down-stream side were also protected simultaneously in accordance with the induced expression of the HSP70-1 gene. Gel mobility shift assays with nuclear extracts from rat liver at 30 min after heat treatment showed maximal DNA binding activity equally with oligonucleotides containing HSE-1 and HSE-2, respectively, and also demonstrated the participation of heat shock factor-1 (HSF1) in both cases. These results suggest that, in addition to HSF1, other regulatory factors might be involved in the dominant protection of HSE-2 in the promoter of the HSP70-1 gene in rat liver after whole body hyperthermia.

Dominant role of the second heat shock element in expression of the HSP70-1 gene in rat liver after whole body hyperthermia. Publishing Authors By Initials

T Konishi,M Nomoto,K Shimizu,T Abe,H Itoh,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research

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Dominant role of the second heat shock element in expression of the HSP70-1 gene in rat liver after whole body hyperthermia. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 118

Page Numbers: 1021-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1995

Dominant role of the second heat shock element in expression of the HSP70-1 gene in rat liver after whole body hyperthermia. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Dominant role of the second heat shock element in expression of the HSP70-1 gene in rat liver after whole body hyperthermia. Keywords Mesh Terms:

KEYWORDS: Rats, Sprague-Dawley

MESH TERMS: metabolism

Chemical & Substance for Abstract: Dominant role of the second heat shock element in expression of the HSP70-1 gene in rat liver after whole body hyperthermia. Information

Substance Name: HSP70 Heat-Shock Proteins

Registry Number: 0

Grant and Affiliation Information for Dominant role of the second heat shock element in expression of the HSP70-1 gene in rat liver after whole body hyperthermia.

AFFILIATION: Department of Biochemistry, University of Occupational and Environmental Health, Yahatanishi-ku, Kitakyushu.

Country: JAPAN

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MEDLINETA: J Biochem

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