Dominant role of betagamma subunits of G-proteins in oxytocin-evoked burst firing.

Dominant role of betagamma subunits of G-proteins in oxytocin-evoked burst firing. Research Abstract Details 

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Dominant role of betagamma subunits of G-proteins in oxytocin-evoked burst firing. Abstract Text:

Yu-Feng Wang,Glenn I Hatton,

Pulsatile neuropeptide secretion is associated with burst firing patterns; however, intracellular signaling cascades leading to bursts remain unclear. We explored mechanisms underlying burst firing in oxytocin (OT) neurons in the supraoptic nucleus in brain slices from lactating rats. Application of 10 pm OT for 30 min or progressively rising OT concentrations from 1 to 100 pm induced burst firing in OT neurons in patch-clamp recordings. Burst generation was blocked by OT antagonist and ionotropic glutamate receptor blockers or tetanus toxin. Blocking G-protein activation with suramin or intracellular GDP-beta-S, but not intracellularly administered antibody against the OT-receptor (OTR) C terminus, blocked bursts. Moreover, pretreatment of slices with pertussis toxin, an inhibitor of G(i/o)-proteins, did not block OT-evoked bursts, suggesting that G(i)/G(o) activation is unnecessary for burst generation. Thus, we further examined G alpha(q/11)-associated signaling pathways in OT-evoked bursts. Inhibition of phospholipase C or RhoA/Rho kinase did not block bursts. Activation of G betagamma subunits using myristoylated G betagamma-binding peptide (mSIRK) caused bursts, whereas intracellularly loaded antibody against G beta subunit blocked OT-evoked bursts. Blocking Src family kinase, but not phosphatidylinositol 3-kinase, occluded OT-evoked bursts. Similar to the effects of OT on EPSCs, mSIRK inhibited tonic EPSCs and elicited EPSC clustering. Finally, suckling caused dissociation of OTRs and G beta subunits from G alpha(q/11) subunits shown by coimmunoprecipitation and immunocytochemistry, supporting crucial roles for OTRs and G betagamma subunits in the milk-ejection reflex. We conclude that G betagamma subunits play a dominant role in burst firing evoked by applied OT or by suckling.

Dominant role of betagamma subunits of G-proteins in oxytocin-evoked burst firing. Publishing Authors By Initials

YF Wang,GI Hatton,

For similar synaptic transmission research abstracts see: synaptic transmission research

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Dominant role of betagamma subunits of G-proteins in oxytocin-evoked burst firing. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of neuroscience : the official journal

VOLUME: 27

Page Numbers: 1902-12

Journal Abbreviation: J. Neurosci.

ISSN: 1529-2401

DAY: 21

MONTH: Feb

YEAR: 2007

Dominant role of betagamma subunits of G-proteins in oxytocin-evoked burst firing. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8102140

Dominant role of betagamma subunits of G-proteins in oxytocin-evoked burst firing. Keywords Mesh Terms:

KEYWORDS: Synaptic Transmission

MESH TERMS: physiology

Chemical & Substance for Abstract: Dominant role of betagamma subunits of G-proteins in oxytocin-evoked burst firing. Information

Substance Name: GTP-Binding Protein alpha Subunits, Gq-G

Registry Number: EC 3.6.1.46

Grant and Affiliation Information for Dominant role of betagamma subunits of G-proteins in oxytocin-evoked burst firing.

AFFILIATION: Department of Cell Biology and Neuroscience, University of California, Riverside, Riverside, California 92521, USA. yufengw@ucr.edu

Country: United States

AGENCY: United States NINDS

GRANT: NS009140

ACRONYM: NS

MEDLINETA: J Neurosci

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