Dodecamer rotor ring defines H+/ATP ratio for ATP synthesis of prokaryotic V-ATPase from Thermus thermophilus.

Dodecamer rotor ring defines H+/ATP ratio for ATP synthesis of prokaryotic V-ATPase from Thermus thermophilus. Research Abstract Details 

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Dodecamer rotor ring defines H+/ATP ratio for ATP synthesis of prokaryotic V-ATPase from Thermus thermophilus. Abstract Text:

Masashi Toei,Christoph Gerle,Masahiro Nakano,Kazutoshi Tani,Nobuhiko Gyobu,Masatada Tamakoshi,Nobuhito Sone,Masasuke Yoshida,Yoshinori Fujiyoshi,Kaoru Mitsuoka,Ken Yokoyama,Masashi Toei,Christoph Gerle,Masahiro Nakano,Kazutoshi Tani,Nobuhiko Gyobu,Masatada Tamakoshi,Nobuhito Sone,Masasuke Yoshida,Yoshinori Fujiyoshi,Kaoru Mitsuoka,Ken Yokoyama,

ATP synthesis by V-ATPase from the thermophilic bacterium Thermus thermophilus driven by the acid-base transition was investigated. The rate of ATP synthesis increased in parallel with the increase in proton motive force (PMF) >110 mV, which is composed of a difference in proton concentration (DeltapH) and the electrical potential differences (DeltaPsi) across membranes. The optimum rate of synthesis reached 85 s(-1), and the H(+)/ATP ratio of 4.0 +/- 0.1 was obtained. ATP was synthesized at a considerable rate solely by DeltapH, indicating DeltaPsi was not absolutely required for synthesis. Consistent with the H(+)/ATP ratio, cryoelectron micrograph images of 2D crystals of the membrane-bound rotor ring of the V-ATPase at 7.0-A resolution showed the presence of 12 V(o)-c subunits, each composed of two transmembrane helices. These results indicate that symmetry mismatch between the rotor and catalytic domains is not obligatory for rotary ATPases/synthases.

Dodecamer rotor ring defines H+/ATP ratio for ATP synthesis of prokaryotic V-ATPase from Thermus thermophilus. Publishing Authors By Initials

M Toei,C Gerle,M Nakano,K Tani,N Gyobu,M Tamakoshi,N Sone,M Yoshida,Y Fujiyoshi,K Mitsuoka,K Yokoyama,M Toei,C Gerle,M Nakano,K Tani,N Gyobu,M Tamakoshi,N Sone,M Yoshida,Y Fujiyoshi,K Mitsuoka,K Yokoyama,

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Dodecamer rotor ring defines H+/ATP ratio for ATP synthesis of prokaryotic V-ATPase from Thermus thermophilus. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 20256-61

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 1091-6490

DAY: 11

MONTH: 12

YEAR: 2007

Dodecamer rotor ring defines H+/ATP ratio for ATP synthesis of prokaryotic V-ATPase from Thermus thermophilus. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

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Grant and Affiliation Information for Dodecamer rotor ring defines H+/ATP ratio for ATP synthesis of prokaryotic V-ATPase from Thermus thermophilus.

AFFILIATION: Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8503, Japan.

Country: United States

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MEDLINETA: Proc Natl Acad Sci U S A

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