DNA vaccine construct incorporating intercellular trafficking and intracellular targeting motifs effectively primes and induces memory B- and T-cell responses in outbred animals.

DNA vaccine construct incorporating intercellular trafficking and intracellular targeting motifs effectively primes and induces memory B- and T-cell responses in outbred animals. Research Abstract Details 

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DNA vaccine construct incorporating intercellular trafficking and intracellular targeting motifs effectively primes and induces memory B- and T-cell responses in outbred animals. Abstract Text:

Waithaka Mwangi,Wendy C Brown,Gary A Splitter,Christopher J Davies,Chris J Howard,Jayne C Hope,Yoko Aida,Yan Zhuang,Beverly J Hunter,Guy H Palmer,

We developed a vaccine construct in which a BVP22 domain and an invariant-chain major histocompatibility complex class II-targeting motif capable of enhancing dendritic cell antigen uptake and presentation were fused to a sequence encoding a B- and T-cell antigen from the Anaplasma marginale major surface protein 1a and tested whether this construct would prime and expand immune responses in outbred calves. A single inoculation with this construct effectively primed the immune responses, as demonstrated by a significant enhancement of CD4(+) T-cell proliferation compared to that in calves identically inoculated but inoculated with a DNA construct lacking the targeting domains and compared to that in calves inoculated with an empty vector. These proliferative responses were mirrored by priming and expansion of gamma interferon-positive CD4(+) T cells and immunoglobulin G responses against the linked B-cell epitope. Priming by the single immunization induced memory that underwent rapid recall following reexposure to the antigen. These results demonstrate that DNA vaccines targeting key intercellular and intracellular events significantly enhance priming and expansion and support the feasibility of single-dose DNA immunization in outbred populations.

DNA vaccine construct incorporating intercellular trafficking and intracellular targeting motifs effectively primes and induces memory B- and T-cell responses in outbred animals. Publishing Authors By Initials

W Mwangi,WC Brown,GA Splitter,CJ Davies,CJ Howard,JC Hope,Y Aida,Y Zhuang,BJ Hunter,GH Palmer,

For similar proteins: recombinant proteins: vaccines, synthetic: vaccines, dna research abstracts see: proteins: recombinant proteins: vaccines, synthetic: vaccines, dna research

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DNA vaccine construct incorporating intercellular trafficking and intracellular targeting motifs effectively primes and induces memory B- and T-cell responses in outbred animals. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Clinical and vaccine immunology : CVI

VOLUME: 14

Page Numbers: 304-11

Journal Abbreviation: Clin. Vaccine Immunol.

ISSN: 1556-6811

DAY: 10

MONTH: 01

YEAR: 2007

DNA vaccine construct incorporating intercellular trafficking and intracellular targeting motifs effectively primes and induces memory B- and T-cell responses in outbred animals. Information

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LANGUAGE: eng

NlmUniqueID: 101252125

DNA vaccine construct incorporating intercellular trafficking and intracellular targeting motifs effectively primes and induces memory B- and T-cell responses in outbred animals. Keywords Mesh Terms:

KEYWORDS: Vaccines, DNA

MESH TERMS: immunology

Chemical & Substance for Abstract: DNA vaccine construct incorporating intercellular trafficking and intracellular targeting motifs effectively primes and induces memory B- and T-cell responses in outbred animals. Information

Substance Name: invariant chain

Registry Number: 0

Grant and Affiliation Information for DNA vaccine construct incorporating intercellular trafficking and intracellular targeting motifs effectively primes and induces memory B- and T-cell responses in outbred animals.

AFFILIATION: Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164, USA. wmwangi@cvm.tamu.edu

Country: United States

AGENCY: United States NIAID

GRANT: T32 AI07025

ACRONYM: AI

MEDLINETA: Clin Vaccine Immunol

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