DNA vaccination against hepatitis E virus infection in cynomolgus macaques.

DNA vaccination against hepatitis E virus infection in cynomolgus macaques. Research Abstract Details 

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DNA vaccination against hepatitis E virus infection in cynomolgus macaques. Abstract Text:

BACKGROUND: : The feasibility of DNA vaccination against hepatitis E in non-human primates has not been evaluated. In the present study a full-length hepatitis E virus (HEV) open reading frame (ORF)2 (Burmese strain) was assembled, cloned, and used for genetic immunization of cynomolgus macaques (cynos), which were subsequently challenged with a heterologous HEV strain (Mexico). METHODS: : Four cynos were vaccinated intramuscularly with the HEV ORF2 DNA cassette and one animal was vaccinated with a mock DNA construct. RESULTS: : Following vaccination anti-HEV antibodies were detected in the four HEV-DNA-vaccinated cynos, but not in the control animal. When challenged, two of the four HEV-DNA-vaccinated cynos were protected against HEV infection and had no elevated alanine aminotransferase activity, viremia, or fecal shedding. The two other DNA-vaccinated animals developed HEV infection and disease. CONCLUSION: : These findings demonstrate the feasibility of DNA vaccination for the protection of HEV infection and warrant further studies to explore routes other than intramuscular for induction of a stronger and efficacious immune response.

DNA vaccination against hepatitis E virus infection in cynomolgus macaques. Publishing Authors By Initials

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DNA vaccination against hepatitis E virus infection in cynomolgus macaques. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of gastroenterology and hepatology

VOLUME: 17 Suppl 3

Page Numbers: S365-9

Journal Abbreviation: J. Gastroenterol. Hepatol.

ISSN: 1440-1746

DAY: 15

MONTH: Dec

YEAR: 2002

DNA vaccination against hepatitis E virus infection in cynomolgus macaques. Information

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LANGUAGE: eng

NlmUniqueID: 8607909

DNA vaccination against hepatitis E virus infection in cynomolgus macaques. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: immunology

Chemical & Substance for Abstract: DNA vaccination against hepatitis E virus infection in cynomolgus macaques. Information

Substance Name: Viral Proteins

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Grant and Affiliation Information for DNA vaccination against hepatitis E virus infection in cynomolgus macaques.

AFFILIATION: Division of Viral Hepatitis, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. skamili@cdc.gov

Country: Australia

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MEDLINETA: J Gastroenterol Hepatol

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