DNA stool test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene.

DNA stool test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene. Research Abstract Details 

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DNA stool test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene. Abstract Text:

Wei Zhang,Michael Bauer,Roland S Croner, Pelz,Dimitri Lodygin,Heiko Hermeking,Michael ,Werner Hohenberger,Klaus E Matzel,Wei Zhang,Michael Bauer,Roland S Croner, Pelz,Dimitri Lodygin,Heiko Hermeking,Michael ,Werner Hohenberger,Klaus E Matzel,

PURPOSE: To investigate a potential mode of noninvasive screening for colorectal cancer, we evaluated the hypermethylation of the secreted frizzled-related protein-1 gene promoter in human stool DNA. METHODS: In stool samples from 36 patients with colorectal neoplasia (7 adenoma, 29 colorectal cancer) and 17 healthy control subjects, isolated DNA was treated with sodium bisulfite and analyzed by methylation-specific polymerase chain reaction with primers specific for methylated or unmethylated promoter sequences of the secreted frizzled-related protein-1 gene. RESULTS: Hypermethylation of the secreted frizzled-related protein-1 promoter was present in the stool DNA of patients with adenoma and colorectal cancer. A sensitivity of 89 percent and specificity of 86 percent were achieved in the detection of colorectal neoplasia. The difference in hypermethylation status of the secreted frizzled-related protein-1 promoter between the patients with colorectal neoplasia and the control group was statistically highly significant (P < 0.001). Adenoma and early tumor Stage I (International Union Against Cancer) displayed both unmethylated and methylated secreted frizzled-related protein-1 promoter sequences, whereas advanced tumor stages showed only methylated secreted frizzled-related protein-1 (P = 0.05). CONCLUSIONS: The results indicate that this DNA stool test of hypermethylation of the secreted frizzled-related protein-1 promoter is a sensitive and specific method. It has the potential of a clinically useful test for the early detection of colorectal cancer.

DNA stool test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene. Publishing Authors By Initials

W Zhang,M Bauer,RS Croner,JO Pelz,D Lodygin,H Hermeking,M ,W Hohenberger,KE Matzel,W Zhang,M Bauer,RS Croner,JO Pelz,D Lodygin,H Hermeking,M ,W Hohenberger,KE Matzel,

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DNA stool test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Diseases of the colon and rectum

VOLUME: 50

Page Numbers: 1618-26; discussion 1626-7

Journal Abbreviation: Dis. Colon Rectum

ISSN: 0012-3706

DAY: 15

MONTH: Oct

YEAR: 2007

DNA stool test for colorectal cancer: hypermethylation of the secreted frizzled-related protein-1 gene. Information

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LANGUAGE: eng

NlmUniqueID: 372764

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AFFILIATION: Department of Surgery, University of Erlangen-Nuremberg, Krankenhausstrasse 12, Erlangen, Germany.

Country: United States

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MEDLINETA: Dis Colon Rectum

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