DNA interstrand cross-linking by epichlorohydrin.

DNA interstrand cross-linking by epichlorohydrin. Research Abstract Details 

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DNA interstrand cross-linking by epichlorohydrin. Abstract Text:

Keith P Romano,Adam G Newman,Rami W Zahran,Julie T Millard,

Epichlorohydrin (ECH), an important industrial chemical, is a bifunctional alkylating agent with the potential to form DNA cross-links. Occupational exposure to this suspect carcinogen leads to chromosomal aberrations, and ECH has been shown previously to undergo reaction with DNA in vivo and in vitro. We used denaturing polyacrylamide gel electrophoresis to monitor the possible formation of interstrand cross-links within DNA oligomers by ECH and the related compound, epibromohydrin (EBH). Although both compounds did indeed form cross-links between deoxyguanosine residues, EBH was a more efficient cross-linker than ECH. The optimal pH for cross-linking also varied, with ECH more efficient at pH 5.0 and EBH more efficient at pH 7.0. Both agents were relatively flexible in the sequences targeted, with comparable efficiencies for 5'-GGC and 5'GC sites. Furthermore, interstrand cross-linking by the two optical isomers of ECH correlated with their relative cytotoxicities, with R-ECH about twice as potent as S-ECH.

DNA interstrand cross-linking by epichlorohydrin. Publishing Authors By Initials

KP Romano,AG Newman,RW Zahran,JT Millard,

For similar natural sciences: chemistry: chemistry, organic: isomerism: stereoisomerism research abstracts see: natural sciences: chemistry: chemistry, organic: isomerism: stereoisomerism research

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DNA interstrand cross-linking by epichlorohydrin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Chemical research in toxicology

VOLUME: 20

Page Numbers: 832-8

Journal Abbreviation: Chem. Res. Toxicol.

ISSN: 0893-228X

DAY: 19

MONTH: 04

YEAR: 2007

DNA interstrand cross-linking by epichlorohydrin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8807448

DNA interstrand cross-linking by epichlorohydrin. Keywords Mesh Terms:

KEYWORDS: Stereoisomerism

MESH TERMS: pathology

Chemical & Substance for Abstract: DNA interstrand cross-linking by epichlorohydrin. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for DNA interstrand cross-linking by epichlorohydrin.

AFFILIATION: Department of Chemistry, Colby College, Waterville Maine 04901, USA.

Country: United States

AGENCY: United States NCI

GRANT: R15 CA077748

ACRONYM: CA

MEDLINETA: Chem Res Toxicol

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