DNA immunisation. New histochemical and morphometric data.

DNA immunisation. New histochemical and morphometric data. Research Abstract Details 

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DNA immunisation. New histochemical and morphometric data. Abstract Text:

Splenic germinal center reactions were measured during primary response to a plasmidic DNA intramuscular injection. Cardiotoxin-pretreated Balb/c mice were immunized with DNA plasmids encodmg or not the SAG1 protein, a membrane antigen of Toxoplasma gondii. Specific anti-SAG1 antibodies were detected on days 16 and 36 after injection of coding plasmids. The results of ELISAs showed that the SAG1-specific antibodies are of the IgG2a class. Morphometric analyses were done on serial immunostained cryosections of spleen and draining or non-draining lymph nodes. This new approach made it possible to evaluate the chronological changes induced by DNA immunisation in the germinal centres (in number and in size). Significant increases in the number of germinal centres were measured in the spleen and only in draining lymph nodes after plasmid injection, the measured changes of the germinal centers appeared to result from the adjuvant stimulatory effect of the plasmidic DNA since both the coding and the noncoding plasmid DNA induced them. No measurable changes were recorded in the T-dependent zone of lymph organs.

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DNA immunisation. New histochemical and morphometric data. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: European journal of histochemistry : EJH

VOLUME: 46

Page Numbers: 215-22

Journal Abbreviation: Eur J Histochem

ISSN: 1121-760X

DAY: 15

MONTH: 08

YEAR: 2002

DNA immunisation. New histochemical and morphometric data. Information

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LANGUAGE: eng

NlmUniqueID: 9207930

DNA immunisation. New histochemical and morphometric data. Keywords Mesh Terms:

KEYWORDS: T-Lymphocytes

MESH TERMS: immunology

Chemical & Substance for Abstract: DNA immunisation. New histochemical and morphometric data. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for DNA immunisation. New histochemical and morphometric data.

AFFILIATION: Laboratory of Human Histology, University of Liège, Belgium. eheinen@ulg.ac.be

Country: Italy

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MEDLINETA: Eur J Histochem

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