DNA fingerprinting of Campylobacter fetus using cloned constructs of ribosomal RNA and surface array protein genes.

DNA fingerprinting of Campylobacter fetus using cloned constructs of ribosomal RNA and surface array protein genes. Research Abstract Details 

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DNA fingerprinting of Campylobacter fetus using cloned constructs of ribosomal RNA and surface array protein genes. Abstract Text:

DNA fragments coding for the ribosomal RNA and the surface array proteins of Campylobacter fetus have been cloned from a genomic library constructed in Escherichia coli. They were used in the molecular characterization of C. fetus (subsp. fetus; subsp. venerealis) strains by restriction fragment length polymorphism (RFLP) method. Ribotyping results showed that all strains of the two subspecies can be classified under one ribogroup implying very close relatedness. The sapA gene DNA marker, however, discriminated all the strains regardless of the subspecies when chromosomal DNA was restricted with HindIII, HaeIII, XbaI or EcoRV. These results illustrate that the sapA probe is potentially useful in fingerprinting C. fetus strains and in determining the relationships of strains for epidemiological purposes.

DNA fingerprinting of Campylobacter fetus using cloned constructs of ribosomal RNA and surface array protein genes. Publishing Authors By Initials

For similar animal diseases: sheep diseases research abstracts see: animal diseases: sheep diseases research

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DNA fingerprinting of Campylobacter fetus using cloned constructs of ribosomal RNA and surface array protein genes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Veterinary microbiology

VOLUME: 54

Page Numbers: 185-93

Journal Abbreviation: Vet. Microbiol.

ISSN: 0378-1135

DAY: 24

MONTH: Feb

YEAR: 1997

DNA fingerprinting of Campylobacter fetus using cloned constructs of ribosomal RNA and surface array protein genes. Information

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LANGUAGE: eng

NlmUniqueID: 7705469

DNA fingerprinting of Campylobacter fetus using cloned constructs of ribosomal RNA and surface array protein genes. Keywords Mesh Terms:

KEYWORDS: Sheep Diseases

MESH TERMS: genetics

Chemical & Substance for Abstract: DNA fingerprinting of Campylobacter fetus using cloned constructs of ribosomal RNA and surface array protein genes. Information

Substance Name: GGCC-specific type II deoxyribonucleases

Registry Number: EC 3.1.21.4

Grant and Affiliation Information for DNA fingerprinting of Campylobacter fetus using cloned constructs of ribosomal RNA and surface array protein genes.

AFFILIATION: Animal Diseases Research Institute Nepean, Ont, Canada.

Country: NETHERLANDS

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MEDLINETA: Vet Microbiol

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