DNA branch nuclease activity of vaccinia a22 resolvase.

DNA branch nuclease activity of vaccinia a22 resolvase. Research Abstract Details 

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DNA branch nuclease activity of vaccinia a22 resolvase. Abstract Text:

Matthew J Culyba,Nana Minkah,Young Hwang,Ori-Michael J Benhamou,Frederic D Bushman,Matthew J Culyba,Nana Minkah,Young Hwang,Ori-Michael J Benhamou,Frederic D Bushman,Matthew J Culyba,Nana Minkah,Young Hwang,Ori-Michael J Benhamou,Frederic D Bushman,

DNA replication, recombination, and repair can result in formation of diverse branched DNA structures. Many large DNA viruses are known to encode DNA branch nucleases, but several of the expected activities have not previously been found among poxvirus enzymes. Vaccinia encodes an enzyme, A22 resolvase, which is known to be active on four-stranded DNA junctions (Holliday junctions) or Holliday junction-like structures containing three of the four strands. Here we report that A22 resolvase in fact has a much wider substrate specificity than previously appreciated. A22 resolvase cleaves Y-junctions, single-stranded DNA flaps, transitions from double strands to unpaired single strands ("splayed duplexes"), and DNA bulges in vitro. We also report site-directed mutagenesis studies of candidate active site residues. The results identify the likely active site and support a model in which a single active site is responsible for cleavage on Holliday junctions and splayed duplexes. Lastly, we describe possible roles for the A22 resolvase DNA-branch nuclease activity in DNA replication and repair.

DNA branch nuclease activity of vaccinia a22 resolvase. Publishing Authors By Initials

MJ Culyba,N Minkah,Y Hwang,OM Benhamou,FD Bushman,MJ Culyba,N Minkah,Y Hwang,OM Benhamou,FD Bushman,MJ Culyba,N Minkah,Y Hwang,OM Benhamou,FD Bushman,

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DNA branch nuclease activity of vaccinia a22 resolvase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 34644-52

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 21

MONTH: 09

YEAR: 2007

DNA branch nuclease activity of vaccinia a22 resolvase. Information

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LANGUAGE: eng

NlmUniqueID: 2985121

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Grant and Affiliation Information for DNA branch nuclease activity of vaccinia a22 resolvase.

AFFILIATION: Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104.

Country: United States

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MEDLINETA: J Biol Chem

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