DNA aptamer-based bioanalysis of IgE by fluorescence anisotropy.

DNA aptamer-based bioanalysis of IgE by fluorescence anisotropy. Research Abstract Details 

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DNA aptamer-based bioanalysis of IgE by fluorescence anisotropy. Abstract Text:

Giridharan Gokulrangan,Jay R Unruh,Douglas F Holub,Brian Ingram,Carey K Johnson,George S Wilson,

A rapid, homogeneous aptamer-based bioanalysis is reported for the sensitive detection of immunoglobulin E (IgE) using fluorescence polarization (FP). 5'-End-labeled D17.4 DNA aptamer was used for IgE detection based on the anisotropy differences of the labeled ligand. Two different fluorophores, fluorescein and Texas Red, were used to analyze IgE in the low-nanomolar range with high specificity. Measurable anisotropy changes were observed with a short equilibration time. Analysis of the binding data reveals a possible cooperative binding process in solution. The nature of the fluorophore clearly influences the sensitivity of the analysis more than the tether length used for the dye conjugation. The local fluorophore motion is seen to influence the sensitivity of the FP probe significantly. Texas Red is seen to be relatively more sensitive for this approach and has apparently favorable dye-DNA interactions, and a limit of detection of 350 pM was obtained. Significant temperature dependence of the FP responses has been observed in this work. Ionic composition of the binding buffer also influences the assay sensitivity. The results confirm the promise and potential of similar homogeneous assays for aptamer-based bioanalysis.

DNA aptamer-based bioanalysis of IgE by fluorescence anisotropy. Publishing Authors By Initials

G Gokulrangan,JR Unruh,DF Holub,B Ingram,CK Johnson,GS Wilson,

For similar heterocyclic compounds: heterocyclic compounds, 3-ring: xanthenes research abstracts see: heterocyclic compounds: heterocyclic compounds, 3-ring: xanthenes research

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DNA aptamer-based bioanalysis of IgE by fluorescence anisotropy. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Analytical chemistry

VOLUME: 77

Page Numbers: 1963-70

Journal Abbreviation: Anal. Chem.

ISSN: 0003-2700

DAY: 1

MONTH: Apr

YEAR: 2005

DNA aptamer-based bioanalysis of IgE by fluorescence anisotropy. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

DNA aptamer-based bioanalysis of IgE by fluorescence anisotropy. Keywords Mesh Terms:

KEYWORDS: Xanthenes

MESH TERMS: chemistry

Chemical & Substance for Abstract: DNA aptamer-based bioanalysis of IgE by fluorescence anisotropy. Information

Substance Name: Texas red

Registry Number: 82354-19-6

Grant and Affiliation Information for DNA aptamer-based bioanalysis of IgE by fluorescence anisotropy.

AFFILIATION: Department of Chemistry, University of Kansas, Lawrence, Kansas 66045, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: 5 T32 GM08545-09

ACRONYM: GM

MEDLINETA: Anal Chem

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