Diverse histone modifications on histone 3 lysine 9 and their relation to DNA methylation in specifying gene silencing.

Diverse histone modifications on histone 3 lysine 9 and their relation to DNA methylation in specifying gene silencing. Research Abstract Details 

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Diverse histone modifications on histone 3 lysine 9 and their relation to DNA methylation in specifying gene silencing. Abstract Text:

Jiejun Wu,Shu-Huei Wang,Dustin Potter,Joseph C Liu,Laura T Smith,Yue-Zhong Wu,Tim H-M Huang,Christoph Plass,

BACKGROUND: Previous studies of individual genes have shown that in a self-enforcing way, dimethylation at histone 3 lysine 9 (dimethyl-H3K9) and DNA methylation cooperate to maintain a repressive mode of inactive genes. Less clear is whether this cooperation is generalized in mammalian genomes, such as mouse genome. Here we use epigenomic tools to simultaneously interrogate chromatin modifications and DNA methylation in a mouse leukemia cell line, L1210. RESULTS: Histone modifications on H3K9 and DNA methylation in L1210 were profiled by both global CpG island array and custom mouse promoter array analysis. We used chromatin immunoprecipitation microarray (ChIP-chip) to examine acetyl-H3K9 and dimethyl-H3K9. We found that the relative level of acetyl-H3K9 at different chromatin positions has a wider range of distribution than that of dimethyl-H3K9. We then used differential methylation hybridization (DMH) and the restriction landmark genome scanning (RLGS) to analyze the DNA methylation status of the same targets investigated by ChIP-chip. The results of epigenomic profiling, which have been independently confirmed for individual loci, show an inverse relationship between DNA methylation and histone acetylation in regulating gene silencing. In contrast to the previous notion, dimethyl-H3K9 seems to be less distinct in specifying silencing for the genes tested. CONCLUSION: This study demonstrates in L1210 leukemia cells a diverse relationship between histone modifications and DNA methylation in the maintenance of gene silencing. Acetyl-H3K9 shows an inverse relationship between DNA methylation and histone acetylation in regulating gene silencing as expected. However, dimethyl-H3K9 seems to be less distinct in relation to promoter methylation. Meanwhile, a combination of epigenomic tools is of help in understanding the heterogeneity of epigenetic regulation, which may further our vision accumulated from single-gene studies.

Diverse histone modifications on histone 3 lysine 9 and their relation to DNA methylation in specifying gene silencing. Publishing Authors By Initials

J Wu,SH Wang,D Potter,JC Liu,LT Smith,YZ Wu,TH Huang,C Plass,

For similar genetic processes: gene expression regulation: protein modification, translational: protein processing, post-translational research abstracts see: genetic processes: gene expression regulation: protein modification, translational: protein processing, post-translational research

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Diverse histone modifications on histone 3 lysine 9 and their relation to DNA methylation in specifying gene silencing. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC genomics

VOLUME: 8

Page Numbers: 131

Journal Abbreviation: BMC Genomics

ISSN: 1471-2164

DAY: 24

MONTH: 05

YEAR: 2007

Diverse histone modifications on histone 3 lysine 9 and their relation to DNA methylation in specifying gene silencing. Information

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LANGUAGE: eng

NlmUniqueID: 100965258

Diverse histone modifications on histone 3 lysine 9 and their relation to DNA methylation in specifying gene silencing. Keywords Mesh Terms:

KEYWORDS: Protein Processing, Post-Translational

MESH TERMS: metabolism

Chemical & Substance for Abstract: Diverse histone modifications on histone 3 lysine 9 and their relation to DNA methylation in specifying gene silencing. Information

Substance Name: DNA Modification Methylases

Registry Number: EC 2.1.1.-

Grant and Affiliation Information for Diverse histone modifications on histone 3 lysine 9 and their relation to DNA methylation in specifying gene silencing.

AFFILIATION: Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA. Jiejun.Wu@osumc.edu <Jiejun.Wu@osumc.edu>

Country: England

AGENCY: United States NCI

GRANT: U54 CA113001

ACRONYM: CA

MEDLINETA: BMC Genomics

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