Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase.

Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase. Abstract Text:

R Lynn Sherrer,Joanne M L Ho,Dieter ,R Lynn Sherrer,Joanne M L Ho,Dieter ,

Selenocysteine (Sec) biosynthesis in archaea and eukaryotes requires three steps: serylation of tRNA(Sec) by seryl-tRNA synthetase (SerRS), phosphorylation of Ser-tRNA(Sec) by O-phosphoseryl-tRNA(Sec) kinase (PSTK), and conversion of O-phosphoseryl-tRNA(Sec) (Sep-tRNA(Sec)) by Sep-tRNA:Sec-tRNA synthase (SepSecS) to Sec-tRNA(Sec). Although SerRS recognizes both tRNA(Sec) and tRNA(Ser) species, PSTK must discriminate Ser-tRNA(Sec) from Ser-tRNA(Ser). Based on a comparison of the sequences and secondary structures of archaeal tRNA(Sec) and tRNA(Ser), we introduced mutations into Methanococcus maripaludis tRNA(Sec) to investigate how Methanocaldococcus jannaschii PSTK distinguishes tRNA(Sec) from tRNA(Ser). Unlike eukaryotic PSTK, the archaeal enzyme was found to recognize the acceptor stem rather than the length and secondary structure of the D-stem. While the D-arm and T-loop provide minor identity elements, the acceptor stem base pairs G2-C71 and C3-G70 in tRNA(Sec) were crucial for discrimination from tRNA(Ser). Furthermore, the A5-U68 base pair in tRNA(Ser) has some antideterminant properties for PSTK. Transplantation of these identity elements into the tRNA(Ser)(UGA) scaffold resulted in phosphorylation of the chimeric Ser-tRNA. The chimera was able to stimulate the ATPase activity of PSTK albeit at a lower level than tRNA(Sec), whereas tRNA(Ser) did not. Additionally, the seryl moiety of Ser-tRNA(Sec) is not required for enzyme recognition, as PSTK efficiently phosphorylated Thr-tRNA(Sec).

Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase. Publishing Authors By Initials

RL Sherrer,JM Ho,D ,RL Sherrer,JM Ho,D ,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nucleic acids research

VOLUME: 36

Page Numbers: 1871-80

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 11

MONTH: 02

YEAR: 2008

Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 411011

Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase.

AFFILIATION: Department of Molecular Biophysics and Biochemistry and Department of Chemistry, Yale University, New Haven, CT 06520-8114, USA.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Nucleic Acids Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase Related Publications

• Amino acid modifications on tRNA.
• Aminoacyl-tRNAs, the bacterial cell envelope, and antibiotics.
• Assays for transfer RNA-dependent amino acid biosynthesis.
• Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation.
• Co-evolution of the archaeal tRNA-dependent amidotransferase GatCAB with tRNA(Asn).
• Covalent attachment of a fluorescent group to 4-thiouridine in transfer RNA.
• Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O-phosphoseryl-tRNASec kinase.
• Emergence of the universal genetic code imprinted in an RNA record.
• From one amino acid to another: tRNA-dependent amino acid biosynthesis.
• Life without RNase P.
• Methanothermobacter thermautotrophicus tRNA Gln confines the amidotransferase GatCAB to asparaginyl-tRNA Asn formation.
• Natural expansion of the genetic code.
• On the evolution of the tRNA-dependent amidotransferases, GatCAB and GatDE.
• Organization and expression of tRNA genes in Drosophila melanogaster.
• Pyrrolysine is not hardwired for cotranslational insertion at UAG codons.
• Recognition of pyrrolysine tRNA by the Desulfitobacterium hafniense pyrrolysyl-tRNA synthetase.
• The Helicobacter pylori amidotransferase GatCAB is equally efficient in glutamine-dependent transamidation of Asp-tRNAAsn and Glu-tRNAGln.
• The genetic code revisited--four decades after Francis Crick.
• Transfer RNA genes in pieces.
• [The danger of developing drug dependence in adolescents]