Distribution of the collagen IV isoforms in human Bruch's membrane.

Distribution of the collagen IV isoforms in human Bruch's membrane. Research Abstract Details 

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Distribution of the collagen IV isoforms in human Bruch's membrane. Abstract Text:

AIMS: To determine the distribution of the alpha1 to alpha6 chains of type IV collagen in Bruch's membrane of the human posterior pole. METHODS: Cryosections (10 micro m) from 18 human eyes (20 months to 83 years old) were acid treated, blocked with 10% normal goat serum, incubated for 1 hour with monoclonal antibodies against type IV collagen isoform specific peptides at 1:75 dilution, and visualised with an ABC staining kit. RESULTS: In Bruch's membrane, the alpha1(IV) and alpha2(IV) chains were identified in retinal pigment epithelial (10/18 = 55%) and choriocapillaris basement membranes (18/18 = 100%); the alpha3(IV), alpha4(IV), and alpha5(IV) chains were also found in the retinal pigment epithelial basement membrane (13/18 = 72%). In the choroid, the alpha1(IV) and alpha2(IV) chains were detected in the blood vessels (18/18=100%). The alpha6(IV) chain was not identified in any sections. CONCLUSION: The heterogeneous distribution of alpha1-2(IV) and alpha3-5(IV) in Bruch's membrane could give insights into the function of this structure in health, ageing, and diseases such as age related macular degeneration.

Distribution of the collagen IV isoforms in human Bruch's membrane. Publishing Authors By Initials

For similar proteins: protein isoforms research abstracts see: proteins: protein isoforms research

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Distribution of the collagen IV isoforms in human Bruch's membrane. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The British journal of ophthalmology

VOLUME: 87

Page Numbers: 212-5

Journal Abbreviation: Br J Ophthalmol

ISSN: 0007-1161

DAY: 28

MONTH: Feb

YEAR: 2003

Distribution of the collagen IV isoforms in human Bruch's membrane. Information

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LANGUAGE: eng

NlmUniqueID: 421041

Distribution of the collagen IV isoforms in human Bruch's membrane. Keywords Mesh Terms:

KEYWORDS: Protein Isoforms

MESH TERMS: chemistry

Chemical & Substance for Abstract: Distribution of the collagen IV isoforms in human Bruch's membrane. Information

Substance Name: Protein Isoforms

Registry Number: 0

Grant and Affiliation Information for Distribution of the collagen IV isoforms in human Bruch's membrane.

AFFILIATION: Department of Ophthalmology, University of California, Davis, USA.

Country: England

AGENCY: United States NEI

GRANT: EY14005

ACRONYM: EY

MEDLINETA: Br J Ophthalmol

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