Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae.

Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae. Abstract Text:

Yasuko Matsumoto,Tsuyoshi Nakano,Masafumi Yamamoto,Yuko Matsushima-Hibiya,Ken-Ichi Odagiri,Osamu Yata,Kotaro Koyama,Takashi Sugimura,Keiji Wakabayashi,

Cabbage butterflies, Pieris rapae and Pieris brassicae, contain strong cytotoxic proteins, designated as pierisin-1 and -2, against cancer cell lines. These proteins exhibit DNA ADP-ribosylating activity. To determine the distribution of substances with cytotoxicity and DNA ADP-ribosylating activity among other species, crude extracts from 20 species of the family Pieridae were examined for cytotoxicity in HeLa cells and DNA ADP-ribosylating activity. Both activities were detected in extracts from 13 species: subtribes Pierina (Pieris rapae, Pieris canidia, Pieris napi, Pieris melete, Pieris brassicae, Pontia daplidice, and Talbotia naganum), Aporiina (Aporia gigantea, Aporia crataegi, Aporia hippia, and Delias pasithoe), and Appiadina (Appias nero and Appias paulina). All of these extracts contained substances recognized by anti-pierisin-1 antibodies, with a molecular mass of approximately 100 kDa established earlier for pierisin-1. Moreover, sequences containing NAD-binding sites, conserved in ADP-ribosyltransferases, were amplified from genomic DNA from 13 species of butterflies with cytotoxicity and DNA ADP-ribosylating activity by PCR. Extracts from seven species, Appias lyncida, Leptosia nina, Anthocharis scolymus, Eurema hecabe, Catopsilia pomona, Catopsilia scylla, and Colias erate, showed neither cytotoxicity nor DNA ADP-ribosylating activity, and did not contain substances recognized by anti-pierisin-1 antibodies. Sequences containing NAD-binding sites were not amplified from genomic DNA from these seven species. Thus, pierisin-like proteins, showing cytotoxicity and DNA ADP-ribosylating activity, are suggested to be present in the extracts from butterflies not only among the subtribe Pierina, but also among the subtribes Aporiina and Appiadina. These findings offer insight to understanding the nature of DNA ADP-ribosylating activity in the butterfly.

Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae. Publishing Authors By Initials

Y Matsumoto,T Nakano,M Yamamoto,Y Matsushima-Hibiya,K Odagiri,O Yata,K Koyama,T Sugimura,K Wakabayashi,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 105

Page Numbers: 2516-20

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 1091-6490

DAY: 6

MONTH: 02

YEAR: 2008

Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae.

AFFILIATION: Cancer Prevention Basic Research Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Proc Natl Acad Sci U S A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Distribution of cytotoxic and DNA ADP-ribosylating activity in crude extracts from butterflies among the family Pieridae Related Publications

• Attachment-inducing capacities of fish skin epithelial extracts on oncomiracidia of Benedenia seriolae (Monogenea: Capsalidae).
• Axial dispositions and conformations of myosin crossbridges along thick filaments in relaxed and contracting states of vertebrate striated muscles by X-ray fiber diffraction.
• Calcium-induced changes in the location and conformation of troponin in skeletal muscle thin filaments.
• Clinical usefulness of the acetaminophen detection kit((r)) in treating acute poisoning-data from a survey of 28 cases treated at niigata city general hospital-.
• Determination of the protein components of native thin filaments isolated from natural actomyosin: nebulin and alpha-actinin are associated with actin filaments.
• ENZYMATIC HYDROLYSIS OF L-PROLYL-BETA-NAPHTHYLAMIDE AND A COLORIMETRIC ASSAY METHOD FOR PROLYLPEPTIDE HYDROLASE ACTIVITY.
• Electron microscopic studies of myosin molecules from chicken gizzard muscle I: the formation of the intramolecular loop in the myosin tail.
• Electron microscopic studies on myosin molecules from chicken gizzard muscle III. Myosin dimers.
• Enhanced Na+/H+ exchange activity contributes to the pathogenesis of muscular dystrophy via involvement of P2 receptors.
• Enhanced thyroid carcinogenicity of N-nitrosobis(2-oxopropyl)amine in Otsuka Long-Evans Tokushima Fatty rats, a model of type II diabetes mellitus.
• Enzymatic properties of pierisin-1 and its N-terminal domain, a guanine-specific ADP-ribosyltransferase from the cabbage butterfly.
• Fluorescence resonance energy transfer between points on actin and the C-terminal region of tropomyosin in skeletal muscle thin filaments.
• Formation of the myosin.ADP.gallium fluoride complex and its solution structure by small-angle synchrotron X-ray scattering.
• Immunohistochemical localization of NUB1, a synphilin-1-binding protein, in neurodegenerative disorders.
• Methanol traps the troponin-tropomyosin-actin complex in an "off-state".
• Mutagenicity of surface soil from residential areas in Kyoto city, Japan, and identification of major mutagens.
• Mutarotation of hydrolysis products by different types of exo-cellulases from Trichoderma viride.
• New method to prepare single-headed heavy meromyosin with high purity and a high yield.
• Nucleotide sequence of an essential region for autonomous replication of cloned yeast mitochondrial DNA.
• Optical diffraction studies on the structure of troponin-tropomyosin-actin paracrystals.
• Relationships among alcohol drinking, blood pressure and serum cholesterol in healthy young women.
• Relocation of Cys374 of actin induced by labeling with fluorescent dyes.
• Solution structure of myosin-ADP-MgFn ternary complex by fluorescent probes and small-angle synchrotron X-ray scattering.
• Three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. I. Tilt angle of myosin subfragment-1 in the rigor complex.
• Three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. III. The multi-domain structure of actin-heavy meromyosin complex.
• Three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. IV. Reconstitution from minimal- and high-dose images of the actin-tropomyosin-myosin subfragment-1 complex.
• Three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. V. Assignment of actin in the actin-tropomyosin-myosin subfragment-1 complex.
• Three-dimensional stress analysis of tooth/lmplant-retained long-span fixed dentures.
• Two autonomously replicating sequences near oli-1 gene of yeast mitochondrial DNA.
• [Family influences on future smoking habits among junior high school students in Japan]