Distinctive epidermal growth factor receptor/extracellular regulated kinase-independent and -dependent signaling pathways in the induction of airway mucin 5B and mucin 5AC expression by phorbol 12-myristate 13-acetate.

Distinctive epidermal growth factor receptor/extracellular regulated kinase-independent and -dependent signaling pathways in the induction of airway mucin 5B and mucin 5AC expression by phorbol 12-myristate 13-acetate. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Distinctive epidermal growth factor receptor/extracellular regulated kinase-independent and -dependent signaling pathways in the induction of airway mucin 5B and mucin 5AC expression by phorbol 12-myristate 13-acetate. Abstract Text:

Daphne Yuan-Chen Wu,Reen Wu,Sekhar P Reddy,Yong Chan Lee,Mary Mann-Jong Chang,

Elevated expression of gel-forming mucin (MUC) genes MUC5AC and MUC5B is a major pathological feature in various airway diseases. In this study, we show that phorbol 12-myristate 13-acetate (PMA) is a potent stimulator for MUC5B gene expression under air-liquid interface conditions in three airway epithelial cell systems: primary cultures of normal human bronchial epithelial cells, the immortalized normal bronchial epithelial cell line HBE1, and the human lung adenocarcinoma cell line A549. Stimulation was time- and dose-dependent, could be demonstrated by promoter-reporter gene transfection, and was sensitive to mithramycin A, suggesting the involvement of a specificity protein 1-based transcriptional mechanism in the stimulation. PMA-induced MUC5B message and promoter-reporter gene activity were specifically sensitive to inhibition of protein kinase C delta, which was further confirmed by the forced expression of dominant-negative mutant of protein kinase C delta. Regarding downstream transduction, PMA-induced MUC5B expression was sensitive to inhibitors and dominant-negative expression of signaling molecules involved in Ras/mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase1-mediated c-Jun N-terminal kinase and p38 pathways. This contrasted with the inhibition of PMA-induced MUC5AC expression by inhibitors of the Ras/epidermal growth factor receptor/extracellular regulated kinase signaling pathway. These results demonstrate for the first time that PMA-stimulated MUC5AC and MUC5B expressions are regulated through distinctive epidermal growth factor receptor/extracellular regulated kinase-dependent and -independent signaling pathways.

Distinctive epidermal growth factor receptor/extracellular regulated kinase-independent and -dependent signaling pathways in the induction of airway mucin 5B and mucin 5AC expression by phorbol 12-myristate 13-acetate. Publishing Authors By Initials

D Yuan-Chen Wu,R Wu,SP Reddy,YC Lee,MM Chang,

For similar respiratory system: trachea research abstracts see: respiratory system: trachea research

PUBMED ID PMID:

MEDLINE DATE:

Distinctive epidermal growth factor receptor/extracellular regulated kinase-independent and -dependent signaling pathways in the induction of airway mucin 5B and mucin 5AC expression by phorbol 12-myristate 13-acetate. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The American journal of pathology

VOLUME: 170

Page Numbers: 20-32

Journal Abbreviation: Am. J. Pathol.

ISSN: 0002-9440

DAY: 3

MONTH: Jan

YEAR: 2007

Distinctive epidermal growth factor receptor/extracellular regulated kinase-independent and -dependent signaling pathways in the induction of airway mucin 5B and mucin 5AC expression by phorbol 12-myristate 13-acetate. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370502

Distinctive epidermal growth factor receptor/extracellular regulated kinase-independent and -dependent signaling pathways in the induction of airway mucin 5B and mucin 5AC expression by phorbol 12-myristate 13-acetate. Keywords Mesh Terms:

KEYWORDS: Trachea

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Distinctive epidermal growth factor receptor/extracellular regulated kinase-independent and -dependent signaling pathways in the induction of airway mucin 5B and mucin 5AC expression by phorbol 12-myristate 13-acetate. Information

Substance Name: Extracellular Signal-Regulated MAP Kinas

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Distinctive epidermal growth factor receptor/extracellular regulated kinase-independent and -dependent signaling pathways in the induction of airway mucin 5B and mucin 5AC expression by phorbol 12-myristate 13-acetate.

AFFILIATION: Center for Comparative Respiratory Biology and Medicine, Genome and Biomedical Science Facility, Suite 6510 University of California, Davis, 451 East Health Sciences Dr., Davis, CA 95616, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL73160-02

ACRONYM: HL

MEDLINETA: Am J Pathol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Distinctive epidermal growth factor receptor/extracellular regulated kinase-independent and -dependent signaling pathways in the induction of airway mucin 5B and mucin 5AC expression by phorbol 12-myristate 13-acetate Related Publications

• A FRET-based approach to ratiometric fluorescence detection of hydrogen peroxide.
• A selective fluorescent sensor for detecting lead in living cells.
• A synchrotron radiation beam-position monitor at the Taiwan Light Source.
• Colorimetric identification of carbohydrates by a pH indicator/pH change inducer ensemble.
• Combinatorial synthesis of benzimidazolium dyes and its diversity directed application toward GTP-selective fluorescent chemosensors.
• Complication of an interbody biopolymer graft extruded orally after anterior cervical discectomy and fusion.
• Dedifferentiation? What's next?
• Diagnostic and treatment interventions for hypochondriasis in the neurology setting.
• HIV-1 gp120 up-regulation of the mu opioid receptor in TPA-differentiated HL-60 cells.
• Improvement of generalised dystonia by olanzapine treatment.
• Laparoscopic treatment of colorectal neoplasia.
• Low catheter sepsis rate in a hospital-wide nutrition support service.
• MR imaging of giant intracranial aneurysm.
• Molecular evolution on chiro-inositol dibenzoate using intramolecular acyl migration and selection by phenyl boronic acid.
• Multiple degradation pathways for misfolded mutants of the yeast plasma membrane ATPase, Pma1.
• Native Hawaiian preferences for informed consent and disclosure of results from genetic research.
• Neural control of the release and action of secretin.
• Nutritional assessment using a microcomputer 1. Programme design.
• Overview of prostate-specific membrane antigen.
• Prognostic significance of cytologic features in fine-needle aspiration cytology samples of papillary thyroid carcinoma: preliminary report.
• Propofol hemisuccinate protects neuronal cells from oxidative injury.
• Racial residential segregation and weight status among US adults.
• Regulation of the neurofibromatosis 2 gene promoter expression during embryonic development.
• Silencing of p29 affects DNA damage responses with UV irradiation.
• The commissioning of a flexible low-cost multipurpose X-ray beamline at SRRC.
• Vascular biology and stroke.
• Visual artificial tongue for quantitative metal-cation analysis by an off-the-shelf dye array.
• [Determination of beryllium in geological samples with slurry sampling and probe atomization in graphite furnace atomic absorption spectrometry]
• [Different expression of chaperone interacting protein in normal, scar and chronic ulcer tissues and its relationship with wound healing]
• [Study on the method for the determination of nitric nitrogen, ammoniacal nitrogen and total nitrogen in plant]