Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst.

Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Abstract Text:

The first two lineages to differentiate from a pluripotent cell population during mammalian development are the extraembryonic trophectoderm (TE) and the primitive endoderm (PrE). Whereas the mechanisms of TE specification have been extensively studied, segregation of PrE and the pluripotent epiblast (EPI) has received comparatively little attention. A current model of PrE specification suggests PrE precursors exhibit an apparently random distribution within the inner cell mass of the early blastocyst and then segregate to their final position lining the cavity by the late blastocyst. We have identified platelet-derived growth factor receptor alpha (Pdgfralpha) as an early-expressed protein that is also a marker of the later PrE lineage. By combining live imaging of embryos expressing a histone H2B-GFP fusion protein reporter under the control of Pdgfra regulatory elements with the analysis of lineage-specific markers, we investigated the events leading to PrE and EPI lineage segregation in the mouse, and correlated our findings using an embryo staging system based on total cell number. Before blastocyst formation, lineage-specific factors are expressed in an overlapping manner. Subsequently, a gradual progression towards a mutually exclusive expression of PrE- and EPI-specific markers occurs. Finally, cell sorting is achieved by a variety of cell behaviours and by selective apoptosis.

Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Development (Cambridge, England)

VOLUME: 135

Page Numbers: 3081-91

Journal Abbreviation: Development

ISSN: 0950-1991

DAY: 26

MONTH: Sep

YEAR: 2008

Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8701744

Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst.

AFFILIATION: Developmental Biology Program, Sloan-Kettering Institute, New York, USA.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Development

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst Related Publications

• A cornucopia of delights for the mouse fancier.
• Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst.
• Eomes::GFP-a tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo.
• The endoderm of the mouse embryo arises by dynamic widespread intercalation of embryonic and extraembryonic lineages.
• The multidimensionality of cell behaviors underlying morphogenesis: a case study in ascidians.