Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice.

Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice. Abstract Text:

Li Liu,Yukisato Ishida,Gbolahan Okunade,Gail J Pyne-Geithman,Gary E Shull,Richard J Paul,

We previously showed that plasma membrane Ca(2+)-ATPase (PMCA) activity accounted for 25-30% of relaxation in bladder smooth muscle (8). Among the four PMCA isoforms only PMCA1 and PMCA4 are expressed in smooth muscle. To address the role of these isoforms, we measured cytosolic Ca(2+) ([Ca(2+)](i)) using fura-PE3 and simultaneously measured contractility in bladder smooth muscle from wild-type (WT), Pmca1(+/-), Pmca4(+/-), Pmca4(-/-), and Pmca1(+/-)Pmca4(-/-) mice. There were no differences in basal [Ca(2+)](i) values between bladder preparations. KCl (80 mM) elicited both larger forces (150-190%) and increases in [Ca(2+)](i) (130-180%) in smooth muscle from Pmca1(+/-) and Pmca1(+/-)Pmca4(-/-) bladders than those in WT or Pmca4(-/-). The responses to carbachol (CCh: 10 muM) were also greater in Pmca1(+/-) (120-150%) than in WT bladders. In contrast, the responses in Pmca4(-/-) and Pmca1(+/-)Pmca4(-/-) bladders to CCh were significantly smaller (40-50%) than WT. The rise in half-times of force and [Ca(2+)](i) increases in response to KCl and CCh, and the concomitant half-times of their decrease upon washout of agonist were prolonged in Pmca4(-/-) (130-190%) and Pmca1(+/-)Pmca4(-/-) (120-250%) bladders, but not in Pmca1(+/-) bladders with respect to WT. Our evidence indicates distinct isoform functions with the PMCA1 isoform involved in overall Ca(2+) clearance, while PMCA4 is essential for the [Ca(2+)](i) increase and contractile response to the CCh receptor-mediated signal transduction pathway.

Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice. Publishing Authors By Initials

L Liu,Y Ishida,G Okunade,GJ Pyne-Geithman,GE Shull,RJ Paul,

For similar urogenital system: urinary tract: urinary bladder research abstracts see: urogenital system: urinary tract: urinary bladder research

PUBMED ID PMID:

MEDLINE DATE:

Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Cell physiology

VOLUME: 292

Page Numbers: C423-31

Journal Abbreviation: Am. J. Physiol., Cell Physiol.

ISSN: 0363-6143

DAY: 6

MONTH: 09

YEAR: 2006

Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901225

Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice. Keywords Mesh Terms:

KEYWORDS: Urinary Bladder

MESH TERMS: physiology

Chemical & Substance for Abstract: Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice. Information

Substance Name: Plasma Membrane Calcium-Transporting ATP

Registry Number: EC 3.6.3.8

Grant and Affiliation Information for Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice.

AFFILIATION: Molecular and Cellular Physiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH 45267-0576, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL 66044

ACRONYM: HL

MEDLINETA: Am J Physiol Cell Physiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice Related Publications

• Acute cutaneous T-cell lymphoma transformation during treatment with alemtuzumab.
• An exploration into study design for biomarker identification: issues and recommendations.
• Antibodies to the superantigenic site of HIV-1 gp120: hydrolytic and binding activities of the light chain subunit.
• Biventricular non-compaction and giant left atrial appendage.
• Chemical deterrence of a cyanobacterial metabolite against generalized and specialized grazers.
• Child assessment of dextromethorphan, diphenhydramine, and placebo for nocturnal cough due to upper respiratory infection.
• Clinical-radiological features of fractures in premature infants--a review.
• Contact dermatitis to hydrolyzed wheat protein.
• Distinct roles of PMCA isoforms in Ca2+ homeostasis of bladder smooth muscle: evidence from PMCA gene-ablated mice.
• Ecological impact of solar ultraviolet-B (UV-B: 320-290 nm) radiation on Corynebacterium aquaticum and Xanthomonas sp. colonization on tea phyllosphere in relation to blister blight disease incidence in the field.
• Efficacy and safety of aminoglycoside monotherapy: systematic review and meta-analysis of randomized controlled trials.
• Evidence that scabies mites (Acari: Sarcoptidae) influence production of interleukin-10 and the function of T-regulatory cells (Tr1) in humans.
• Evidence that the deficit in sexual behavior in adult rats neonatally exposed to citalopram is a consequence of 5-HT1 receptor stimulation during development.
• Expression and function of COOH-terminal myosin heavy chain isoforms in mouse smooth muscle.
• Genetic and physiological evidence that oligodendrocyte gap junctions contribute to spatial buffering of potassium released during neuronal activity.
• Higher plasma but not intracellular concentrations after infusion with liposomal daunorubicin compared with conventional daunorubicin in adult acute myeloid leukemia.
• How do the vaccine polio viruses replace the wild polio viruses?
• Identifying cardinal dates in phytoplankton time series to enable the analysis of long-term trends.
• Lymphopenic mice reconstituted with limited repertoire T cells develop severe, multiorgan, Th2-associated inflammatory disease.
• Modelling of biological nitrogen removal during treatment of piggery wastewater.
• Modelling of biological processes during aerobic treatment of piggery wastewater aiming at process optimisation.
• Naphthaquinones of Diospyros batocana.
• New insights in chronic allograft rejection.
• SSPE and measles vaccine.
• Smart brackets for 3D-force-moment measurements in orthodontic research and therapy - developmental status and prospects.
• The effect of micro-CN linkage isomerism and ancillary ligand set on directional metal-metal charge-transfer in cyanide-bridged dimanganese complexes.
• The use of atrial overdrive and ventricular rate stabilization pacing algorithms for the prevention and treatment of paroxysmal atrial fibrillation: the Pacemaker Atrial Fibrillation Suppression (PAFS) study.
• Towards irreversible HIV inactivation: stable gp120 binding by nucleophilic antibodies.
• [In Process Citation]
• [In Process Citation]