Distinct effects of individual opioids on the morphology of spines depend upon the internalization of mu opioid receptors.

Distinct effects of individual opioids on the morphology of spines depend upon the internalization of mu opioid receptors. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Distinct effects of individual opioids on the morphology of spines depend upon the internalization of mu opioid receptors. Abstract Text:

Dezhi Liao,Olga O Grigoriants,Wei Wang,Katie Wiens,Horace H Loh,Ping-Yee Law,

This study has examined the relationship between the effects of opioids on the internalization of mu opioid receptors (MORs) and the morphology of dendritic spines. Several opioids (morphine, etorphine, DAMGO or methadone) were applied to cultured hippocampal neurons. Live imaging and biochemical techniques were used to examine the dynamic changes in MOR internalization and spine morphology. This study reveals that MOR internalization can regulate opioid-induced morphological changes in dendritic spines: (1) Chronic treatment with morphine, which induced minimal receptor internalization, caused collapse of dendritic spines. In contrast, "internalizing" opioids such as DAMGO and etorphine induced the emergence of new spines. It reveals that opioid-induced changes in spines vary greatly depending on how the applied opioid agonist affects MOR internalization. (2) The blockade of receptor internalization by dominant negative mutant of dynamin, K44E, reversed the effects of DAMGO and etorphine. It indicates that receptor internalization is necessary for the distinct effects of DAMGO and etorphine on spines. (3) In neurons that were cultured from MOR knock-out mice and had been co-transfected with DsRed and MOR-GFP, morphine caused collapse of spines whereas DAMGO induced emergence of new spines, indicating that opioids can alter the structure of spines via postsynaptic MORs. (4) Methadone at a low concentration induced minimal internalization and had effects that were similar to morphine. At a high concentration, methadone induced robust internalization and had effects that are opposite to morphine. The concentration-dependent opioid-induced changes in dendritic spines might also contribute to the variation in the effects of individual opioids.

Distinct effects of individual opioids on the morphology of spines depend upon the internalization of mu opioid receptors. Publishing Authors By Initials

D Liao,OO Grigoriants,W Wang,K Wiens,HH Loh,PY Law,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

PUBMED ID PMID:

MEDLINE DATE:

Distinct effects of individual opioids on the morphology of spines depend upon the internalization of mu opioid receptors. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular and cellular neurosciences

VOLUME: 35

Page Numbers: 456-69

Journal Abbreviation: Mol. Cell. Neurosci.

ISSN: 1044-7431

DAY: 4

MONTH: 05

YEAR: 2007

Distinct effects of individual opioids on the morphology of spines depend upon the internalization of mu opioid receptors. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9100095

Distinct effects of individual opioids on the morphology of spines depend upon the internalization of mu opioid receptors. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Distinct effects of individual opioids on the morphology of spines depend upon the internalization of mu opioid receptors. Information

Substance Name: Receptors, Opioid, mu

Registry Number: 0

Grant and Affiliation Information for Distinct effects of individual opioids on the morphology of spines depend upon the internalization of mu opioid receptors.

AFFILIATION: Department of Neuroscience and Basic Research Center on Molecular and Cell Biology of Drug Addiction, The University of Minnesota, 321 Church St SE, Minneapolis, MN 55455, USA. liaox020@tc.umn.edu

Country: United States

AGENCY: United States NIDA

GRANT: R01DA020582

ACRONYM: DA

MEDLINETA: Mol Cell Neurosci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Distinct effects of individual opioids on the morphology of spines depend upon the internalization of mu opioid receptors Related Publications

• -Arrestin-Dependent {micro}-Opioid Receptor-Activated Extracellular Signal-Regulated Kinases (ERKs) Translocate to Nucleus in Contrast to G Protein-Dependent ERK Activation.
• A firm persuasion in our work. Occupational therapy: a journey driven by curiosity.
• Aedes aegypti ferritin heavy chain homologue: feeding of iron or blood influences message levels, lengths and subunit abundance.
• Agonist-dependent postsynaptic effects of opioids on miniature excitatory postsynaptic currents in cultured hippocampal neurons.
• An assessment of chemical pollution from the MSC Napoli.
• An attempt to demonstrate neutralizing antibodies to the mammary tumour milk agent in mice.
• Deep proteome profiling of sera from never-smoked lung cancer patients.
• Differentiating intracranial aspergillosis from a high grade glioma using MRI and MR spectroscopic imaging.
• Distinct effects of individual opioids on the morphology of spines depend upon the internalization of mu opioid receptors.
• Fluid transport across the isolated porcine ciliary epithelium.
• Gs15p is there a better way to determine private surgical fees in new zealand?
• Interview therapy of psychosomatic arthritis.
• Kinematic condition for multicomponent lattice Boltzmann simulation.
• Modelling the hepatitis C virus epidemic in Australia.
• Modelling the population-level impact of vaccination on the transmission of human papillomavirus type 16 in Australia.
• Molecular cloning of a brain-specific, developmentally regulated neuregulin 1 (NRG1) isoform and identification of a functional promoter variant associated with schizophrenia.
• Nonparaxial propagation of a cylindrical beam with Lanczos reduction.
• Novel fatty acids from the royal jelly of honeybees (Apis mellifera, L.).
• Osteotomy of the spine in the treatment of severe dorsal kyphosis; four cases.
• Phosphorylation of the delta-opioid receptor regulates its beta-arrestins selectivity and subsequent receptor internalization and adenylyl cyclase desensitization.
• Propagation of nonparaxial beams with a modified Arnoldi method.
• Ring location in cyclopropane fatty acid esters by a mass spectrometric method.
• Self-reported psychological States and physiological responses to different types of motivational general imagery.
• Some notes on hyperphoria with illustrative case histories.
• The hygiene of the scalp and hair.
• The importance of size and growth in infancy: integrated findings from systematic reviews of scientific evidence and lay perspectives.
• The role of a clinical director in developing an innovative assertive community treatment team targeting ethno-racial minority patients.
• The toxicological impacts of oil and chemically dispersed oil: UV mediated phototoxicity and implications for environmental effects, statutory testing and response strategies.
• Use of chewing gum in reducing postoperative ileus after elective colorectal resection: a systematic review.
• Use of colonic stents in emergent malignant left colonic obstruction: a markov chain monte carlo decision analysis.