Dissociation of m-calpain subunits occurs after autolysis of the N-terminus of the catalytic subunit, and is not required for activation.

Dissociation of m-calpain subunits occurs after autolysis of the N-terminus of the catalytic subunit, and is not required for activation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Dissociation of m-calpain subunits occurs after autolysis of the N-terminus of the catalytic subunit, and is not required for activation. Abstract Text:

K Nakagawa,H Masumoto,H Sorimachi,K Suzuki,

Calpain is a heterodimeric, intracellular Ca(2+)-dependent, "bio-modulator" that alters the properties of substrates through site-specific proteolysis. It has been proposed that calpains are activated by autolysis of the N-terminus of the large subunit and/or its dissociation into the subunits. It is, however, unclear whether the dissociation into subunits is required for the expression of protease activity and/or for in vivo function. Recently, the crystal structure of m-calpain in the absence of Ca(2+) has been resolved. The 3D structure clearly shows that the N-terminus of the m-calpain large subunit (mCL) makes contact with the 30K subunit, suggesting that autolysis of the N-terminus of mCL changes the interaction of both subunits. To examine the relationship between autolysis, dissociation, and activation, we made and analysed a series of N-terminal mutants of mCL that mimic the autolysed forms or have substituted amino acid residue(s) interacting with 30K. As a result, the mutant m-calpains, which are incapable of autolysis, did not dissociate into subunits, whereas those lacking the N-terminal 19 residues (Delta 19), but not those lacking only nine residues (Delta 9), dissociated into subunits even in the absence of Ca(2+). Moreover, both Delta 9 and Delta 19 mutants showed an equivalent reduced Ca(2+) requirement for protease activity. These results indicate that autolysis is necessary for the dissociation of the m-calpain subunits, and that the dissociation occurs after, but is not necessary for, activation.

Dissociation of m-calpain subunits occurs after autolysis of the N-terminus of the catalytic subunit, and is not required for activation. Publishing Authors By Initials

K Nakagawa,H Masumoto,H Sorimachi,K Suzuki,

For similar proteins: protein subunits research abstracts see: proteins: protein subunits research

PUBMED ID PMID:

MEDLINE DATE:

Dissociation of m-calpain subunits occurs after autolysis of the N-terminus of the catalytic subunit, and is not required for activation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 130

Page Numbers: 605-11

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 2001

Dissociation of m-calpain subunits occurs after autolysis of the N-terminus of the catalytic subunit, and is not required for activation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Dissociation of m-calpain subunits occurs after autolysis of the N-terminus of the catalytic subunit, and is not required for activation. Keywords Mesh Terms:

KEYWORDS: Protein Subunits

MESH TERMS: cytology

Chemical & Substance for Abstract: Dissociation of m-calpain subunits occurs after autolysis of the N-terminus of the catalytic subunit, and is not required for activation. Information

Substance Name: Calpain

Registry Number: EC 3.4.22.-

Grant and Affiliation Information for Dissociation of m-calpain subunits occurs after autolysis of the N-terminus of the catalytic subunit, and is not required for activation.

AFFILIATION: Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Dissociation of m-calpain subunits occurs after autolysis of the N-terminus of the catalytic subunit, and is not required for activation Related Publications

• A uniformly cleaved epitaxically grown diamond crystal for synchrotron radiation.
• Absolute soft X-ray measurements using an ion chamber.
• Agmatine suppresses mesangial cell proliferation by modulating polyamine metabolism.
• Attention induces synchronization-based response gain in steady-state visual evoked potentials.
• Behavior of Medaka fish under distributed gravity.
• Branched-chain amino acid supplementation during the endoscopic injection sclerotherapy for esophageal varices may be useful for maintenance of nutritional status in patients with liver cirrhosis.
• Comprehensive genetic selection revealed essential bases in the peptidyl-transferase center.
• Cytokine production of CD8+ immune T cells but not of CD4+ T cells from Toxoplasma gondii-infected mice is polarized to a type 1 response following stimulation with tachyzoite-infected macrophages.
• Developmental capacity of bovine oocytes cryopreserved after maturation in vitro and of frozen-thawed bovine embryos derived from frozen mature oocytes.
• Developmental capacity of bovine oocytes frozen in different cryoprotectants.
• Effects of different lots of semen from the same bull on in vitro development of bovine oocytes fertilized in vitro.
• Effects of pressure on the phase transition of bilayers in liposomes. Influence of cholesterol and alpha-tocopherol.
• Elevated extracellular calcium stimulates secretion of bone morphogenetic protein 2 by a macrophage cell line.
• Expression of bHLH Transcription Factors and IGFs in the Non-sensory Patches, Olfactory Epithelium and Vomeronasal Organ.
• Fasting plasma ghrelin levels reflect malnutrition state in patients with liver cirrhosis.
• Hepatocyte-specific Pten-deficient mice as a novel model for nonalcoholic steatohepatitis and hepatocellular carcinoma.
• Hyperplasia and carcinomas in Pten-deficient mice and reduced PTEN protein in human bladder cancer patients.
• Improvement in cognitive function after radical excision of an anterior skull base meningioma - a report of 2 cases.
• Independent representation of parts and the relations between them: evidence from integrative agnosia.
• Prognosis-predicting equations using histological scores contribute to the choice of steroid therapy in IgA nephropathy: Multivariate analysis on 275 adult patients.
• Proteome analysis of glial cells treated by radiation or hydroperoxide.
• Relationship between progesterone and estradiol contents of follicular fluid and the morphological appearance of granulosa cells of follicles coexisting with corpora lutea in bovine ovaries.
• Safety of ablation of the sigmoid and transverse sinuses: an experimental study.
• Species specific differences in the ratio of short to long loop nephrons in the kidneys of laboratory rodents.
• Superovulation in the cow with a single intramuscular injection of FSH dissolved in polyvinylpyrrolidone.
• Synergism between a mycoreovirus and a hypovirus mediated by the papain-like protease p29 of the prototypic hypovirus CHV1-EP713.
• The A-site finger in 23 S rRNA acts as a functional attenuator for translocation.
• Viability of frozen-thawed bovine IVM/IVF embryos in relation to aging using various cryoprotectants.
• [High performance liquid chromatographic determination of reducing sugars in fruit juices with laser resonance Raman detection]
• [Selective extraction of organotin from biological sample by supercritical fluid extraction]