Disruption of the mouse protein Ser/Thr phosphatase 2Cbeta gene leads to early pre-implantation lethality.

Disruption of the mouse protein Ser/Thr phosphatase 2Cbeta gene leads to early pre-implantation lethality. Research Abstract Details 

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Disruption of the mouse protein Ser/Thr phosphatase 2Cbeta gene leads to early pre-implantation lethality. Abstract Text:

Masato Sasaki,Motoko Ohnishi,Fumi Tashiro,Hitoshi Niwa,Akira Suzuki,Jun-Ichi Miyazaki,Takayasu Kobayashi,Shinri Tamura,

Protein phosphatase 2Cbeta (PP2Cbeta) is a member of a family of protein Ser/Thr phosphatases (PP2C) that is composed of at least twelve different gene products. Recent studies have revealed that PP2Cbeta mRNA accumulates in mature sperm, unfertilized metaphase II-arrested oocytes and zygotes, but that the mRNA level then decreases sharply between the early two-cell and eight-cell stages, remaining at low levels during the 16-cell to blastocyst stages of mice. These observations raised the possibility that PP2Cbeta plays a crucial role during gametogenesis, fertilization, and/or early stages of embryonic development. In this study, we employed a gene knockout technique in mice to test this possibility. We found that PP2Cbeta(Delta/wt) mice generate normal mature gametes. However, PP2Cbeta(Delta/Delta) embryos die between the two-cell and eight-cell stages. To our interest, PP2Cbeta(Delta/Delta) ES cells which had been generated by transfecting PP2Cbeta(3lox/3lox) ES cells with Cre-expressing plasmid were viable. In addition, knockdown of PP2Cbeta using siRNA did not affect the proliferation of wild-type ES cells. These observations suggest that relatively high PP2Cbeta expression is specifically required during the early stages of pre-implantation development. The possible mechanisms for the early pre-implantation lethality of PP2Cbeta(Delta/Delta) mice are discussed.

Disruption of the mouse protein Ser/Thr phosphatase 2Cbeta gene leads to early pre-implantation lethality. Publishing Authors By Initials

M Sasaki,M Ohnishi,F Tashiro,H Niwa,A Suzuki,J Miyazaki,T Kobayashi,S Tamura,

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Disruption of the mouse protein Ser/Thr phosphatase 2Cbeta gene leads to early pre-implantation lethality. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Mechanisms of development

VOLUME: 124

Page Numbers: 489-99

Journal Abbreviation: Mech. Dev.

ISSN: 0925-4773

DAY: 11

MONTH: 04

YEAR: 2007

Disruption of the mouse protein Ser/Thr phosphatase 2Cbeta gene leads to early pre-implantation lethality. Information

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LANGUAGE: eng

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AFFILIATION: Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai 980-8575, Japan; Department of Molecular Biology, Akita University School of Medicine, Akita 010-8543, Japan.

Country: Ireland

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MEDLINETA: Mech Dev

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