Disparate mechanisms of sICAM-1 production in the peripheral lung: contrast between alveolar epithelial cells and pulmonary microvascular endothelial cells.

Disparate mechanisms of sICAM-1 production in the peripheral lung: contrast between alveolar epithelial cells and pulmonary microvascular endothelial cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Disparate mechanisms of sICAM-1 production in the peripheral lung: contrast between alveolar epithelial cells and pulmonary microvascular endothelial cells. Abstract Text:

Membrane-associated intercellular adhesion molecule-1 (mICAM-1; CD54) is constitutively expressed on the surface of type I alveolar epithelial cells (AEC). Soluble ICAM-1 (sICAM-1) may be produced by proteolytic cleavage of mICAM-1 or by alternative splicing of ICAM-1 mRNA. In contrast to inducible expression seen in most cell types, sICAM-1 is constitutively released by type I AEC and is present in normal alveolar lining fluid. Therefore, we compared the mechanism of sICAM-1 production in primary cultures of two closely juxtaposed cells in the alveolar wall, AEC and pulmonary microvascular endothelial cells (PVEC). AEC, but not PVEC, demonstrated high-level baseline expression of sICAM-1. Stimulation of AEC with TNFalpha or LPS resulted in minimal increase in AEC sICAM-1, whereas PVEC sICAM-1 was briskly induced in response to these signals. AEC sICAM-1 shedding was significantly reduced by treatment with a serine protease inhibitor, but not by cysteine, metalloprotease, or aspartic protease inhibitors. In contrast, none of these inhibitors effected sICAM-1 expression in PVEC. RT-PCR, followed by gel analysis of total RNA, suggests that alternatively spliced fragments are present in both cell types. However, a 16-mer oligopeptide corresponding to the juxtamembrane region of mICAM-1 completely abrogated sICAM-1 shedding in AEC but reduced stimulated PVEC sICAM-1 release by only 20%. Based on these data, we conclude that the predominant mechanism of sICAM-1 production likely differs in the two cell types from opposite sides of the alveolar wall.

Disparate mechanisms of sICAM-1 production in the peripheral lung: contrast between alveolar epithelial cells and pulmonary microvascular endothelial cells. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Disparate mechanisms of sICAM-1 production in the peripheral lung: contrast between alveolar epithelial cells and pulmonary microvascular endothelial cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: American journal of physiology. Lung cellular and

VOLUME: 294

Page Numbers: L807-14

Journal Abbreviation: Am. J. Physiol. Lung Cell Mol.

ISSN: 1040-0605

DAY: 15

MONTH: 02

YEAR: 2008

Disparate mechanisms of sICAM-1 production in the peripheral lung: contrast between alveolar epithelial cells and pulmonary microvascular endothelial cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901229

Disparate mechanisms of sICAM-1 production in the peripheral lung: contrast between alveolar epithelial cells and pulmonary microvascular endothelial cells. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Disparate mechanisms of sICAM-1 production in the peripheral lung: contrast between alveolar epithelial cells and pulmonary microvascular endothelial cells. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Disparate mechanisms of sICAM-1 production in the peripheral lung: contrast between alveolar epithelial cells and pulmonary microvascular endothelial cells.

AFFILIATION: Pulmonary Section (111G Veterans Affairs Medical Center, 2215 Fuller Road, Ann Arbor, MI 48105. mpmendez@umich.edu).

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Am J Physiol Lung Cell Mol Phy

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Disparate mechanisms of sICAM-1 production in the peripheral lung: contrast between alveolar epithelial cells and pulmonary microvascular endothelial cells Related Publications

• A case of masculinizing adrenal tumour.
• Achieving "organic compositionality" through self-organization: Reviews on brain-inspired robotics experiments.
• Aureomycin; a new antibiotic; evaluation of its effects in typhoid fever, severe salmonella infections and in a case of colon bacillus bacteremia.
• Bilateral symmetrical retinal cysts.
• Carcinoma of the esophagus; report of cases: 1947 to 1953.
• Cellular uptake of amelogenin, and its localization to CD63, and Lamp1-positive vesicles.
• Characterization of inhibitors and substrates of Anopheles gambiae CYP6Z2.
• Comparative hospital costs.
• Concise synthesis of the tricyclic core of lycoposerramine S.
• Continuous suction drainage; a historical evaluation of intestinal obstruction.
• Determination of protein regions responsible for interactions of amelogenin with CD63 and LAMP1.
• Disparate mechanisms of sICAM-1 production in the peripheral lung: contrast between alveolar epithelial cells and pulmonary microvascular endothelial cells.
• Esters of Pyromellitic Acid. Part II. Esters of Chiral Alcohols: Para Pyromellitate Diesters as a Novel Class of Resolving Agents and Use of Pyromellitates as Duplicands for Chiral Purification.
• Esters of pyromellitic Acid. Part I. Esters of achiral alcohols: regioselective synthesis of partial and mixed pyromellitate esters, mechanism of transesterification in the quantitative esterification of the pyromellitate system using orthoformate esters,
• GM-CSF and the impaired pulmonary innate immune response following hyperoxic stress.
• General report and commentary: the realities of quality assurance.
• Human enteric microsomal CYP4F enzymes O-demethylate the antiparasitic prodrug pafuramidine.
• Looking forward in oncology.
• Oxidation Reactivity Channels for 2-(Pyridin-2-yl)-N,N-diphenylacetamides.
• Poliomyelitis in Berlin.
• Present outlook in the treatment of carcinoma of the lower esophagus and cardia of the stomach.
• Pulse-wave velocity as an index of aging in the cardiovascular system.
• REVIEW OF NECROPSIES, MEDICAL SERVICE, LOS ANGELES GENERAL HOSPITAL.
• Sildenafil treatment of women with antidepressant-associated sexual dysfunction: a randomized controlled trial.
• Studies of ventricular fibrillation caused by electric shock: II. Cinematographic and electrocardiographic observations of the natural process in the dog's heart. Its inhibition by potassium and the revival of coordinated beats by calcium.
• The Influence of CYP3A5 Expression on the Extent of Hepatic CYP3A Inhibition Is Substrate-Dependent: An in Vitro-in Vivo Evaluation.
• The surgery of mitral valvular disease.
• Training in urology.
• Tree recruitment in an empty forest.
• Vestibular rehabilitation in individuals with inner-ear dysfunction: a pilot study.