Discrimination of sheep susceptible and resistant to transmissible spongiform encephalopathies by an haplotype specific monoclonal antibody.

Discrimination of sheep susceptible and resistant to transmissible spongiform encephalopathies by an haplotype specific monoclonal antibody. Research Abstract Details 

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Discrimination of sheep susceptible and resistant to transmissible spongiform encephalopathies by an haplotype specific monoclonal antibody. Abstract Text:

J M Bilheude,A Brun,N Morel,F ,S Lecroix,J C Espinosa,L ,P Steele,J Grassi,O ,J M Torres,J M Bilheude,A Brun,N Morel,F ,S Lecroix,J C Espinosa,L ,P Steele,J Grassi,O ,J M Torres,

In the present report, the selective detection of sheep PrP haplotypes by monoclonal antibody 2A11 is described. It is showed that the substitution of glutamine by arginine but not by histidine at ovine PrP position 171 abolishes completely the recognition of either PrP(c) or PrP(d) by mAb 2A11, in such a way that the application of this antibody allows the unambiguous discrimination of R(171) homozygotes. On the basis of the high resistance to classical scrapie and bovine spongiform encephalophaty (BSE) infection associated to the R(171) PrP haplotype, animals bearing the ARR allele are currently selected within the scrapie national plan initiated in Great Britain. A 2A11-based immuno enzymatic test have been developed and evaluated using a panel of plasma and sera from sheep of different PrP genotypes and breeds. The test allows the efficient discrimination of R(171) homozygotes, R(171) heterozygotes and non-R(171) carriers, therefore offering a rapid, cheap and easy to use alternative method to select sheep for their resistance to scrapie.

Discrimination of sheep susceptible and resistant to transmissible spongiform encephalopathies by an haplotype specific monoclonal antibody. Publishing Authors By Initials

JM Bilheude,A Brun,N Morel,F ,S Lecroix,JC Espinosa,L ,P Steele,J Grassi,O ,JM Torres,JM Bilheude,A Brun,N Morel,F ,S Lecroix,JC Espinosa,L ,P Steele,J Grassi,O ,JM Torres,

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Discrimination of sheep susceptible and resistant to transmissible spongiform encephalopathies by an haplotype specific monoclonal antibody. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of virological methods

VOLUME: 145

Page Numbers: 169-72

Journal Abbreviation: J. Virol. Methods

ISSN: 0166-0934

DAY: 5

MONTH: 07

YEAR: 2007

Discrimination of sheep susceptible and resistant to transmissible spongiform encephalopathies by an haplotype specific monoclonal antibody. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Discrimination of sheep susceptible and resistant to transmissible spongiform encephalopathies by an haplotype specific monoclonal antibody.

AFFILIATION: Bio-Rad, R&D TSE, 3 Bd Raymond Poincaré, 92430 Marnes La Coquette, France.

Country: Netherlands

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MEDLINETA: J Virol Methods

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