Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Research Abstract Details 

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Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Abstract Text:

Johanna E Camara,Faith A Hays,Johanna E Camara,Faith A Hays,

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was optimized to discriminate between wild-type and ampicillin-resistant Escherichia coli. Only ampicillin-resistant E. coli displayed an m/z approximately 29,000 peak, which was confirmed as beta-lactamase by in-gel digestion followed by peptide mass fingerprinting. Rapid MALDI-TOF MS detection of antibiotic-resistance could fulfill an important clinical need, providing critical phenotypic information beyond genus-species identification.

Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Publishing Authors By Initials

JE Camara,FA Hays,JE Camara,FA Hays,

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Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical and bioanalytical chemistry

VOLUME: 389

Page Numbers: 1633-8

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ISSN: 1618-2642

DAY: 12

MONTH: 09

YEAR: 2007

Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Information

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LANGUAGE: eng

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AFFILIATION: Analytical Chemistry Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD 20899, USA. johanna.camara@nist.gov

Country: Germany

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MEDLINETA: Anal Bioanal Chem

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