Disappearance of guanosine 5'-diphosphate 3'-diphosphate in Bacillus subtilis vegetative cells upon carbon source deprivation.

Disappearance of guanosine 5'-diphosphate 3'-diphosphate in Bacillus subtilis vegetative cells upon carbon source deprivation. Research Abstract Details 

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Disappearance of guanosine 5'-diphosphate 3'-diphosphate in Bacillus subtilis vegetative cells upon carbon source deprivation. Abstract Text:

K Ikehara,K Maeda,S Makino,K Sugae,

Bacillus subtilis was grown in a nutrient medium, mNSMP, and a synthetic medium, mS6(C), in which spore formation was initiated after vegetative growth and exhaustion of carbon source or glucose. The amounts of intracellular phosphorylated compounds were analyzed at intervals by 2 M formic acid extraction and polyethyleneimine (PEI)-cellulose thin-layer chromatography followed by autoradiography. A hyperphosphorylated nucleotide, guanosine 5'-diphosphate 3'-diphosphate (ppGpp), was accumulated in cells during vegetative growth in both mNSMP and mS6(C), and then the nucleotide was degraded upon initiation of sporulation in both cases. Furthermore, after the nucleotide had disappeared in cells cultivated in mS6(C) upon exhaustion of the carbon source, it could be reformed in the sporulating cells by addition of glucose to the medium. These results suggest that the ppGpp in vegetative cells may function in the regulation of B. subtilis sporulation.

Disappearance of guanosine 5'-diphosphate 3'-diphosphate in Bacillus subtilis vegetative cells upon carbon source deprivation. Publishing Authors By Initials

K Ikehara,K Maeda,S Makino,K Sugae,

For similar cells: spores: spores, bacterial research abstracts see: cells: spores: spores, bacterial research

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Disappearance of guanosine 5'-diphosphate 3'-diphosphate in Bacillus subtilis vegetative cells upon carbon source deprivation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 89

Page Numbers: 517-21

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1981

Disappearance of guanosine 5'-diphosphate 3'-diphosphate in Bacillus subtilis vegetative cells upon carbon source deprivation. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Disappearance of guanosine 5'-diphosphate 3'-diphosphate in Bacillus subtilis vegetative cells upon carbon source deprivation. Keywords Mesh Terms:

KEYWORDS: Spores, Bacterial

MESH TERMS: physiology

Chemical & Substance for Abstract: Disappearance of guanosine 5'-diphosphate 3'-diphosphate in Bacillus subtilis vegetative cells upon carbon source deprivation. Information

Substance Name: Carbon

Registry Number: 7440-44-0

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Country: JAPAN

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MEDLINETA: J Biochem

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Disappearance of guanosine 5'-diphosphate 3'-diphosphate in Bacillus subtilis vegetative cells upon carbon source deprivation Related Publications

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• Induction of Bacillus subtilis sporulation by decoyinine and the concomitant disappearance of ppGpp in vegetative cells.
• On the C-terminal amino acid of bacterial alpha-amylase.
• Presence of guanosine 5'-diphosphate 3'-diphosphate in Bacillus subtilis vegetative cells.