Directional shear flow and Rho activation prevent the endothelial cell apoptosis induced by micropatterned anisotropic geometry.

Directional shear flow and Rho activation prevent the endothelial cell apoptosis induced by micropatterned anisotropic geometry. Research Abstract Details 

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Directional shear flow and Rho activation prevent the endothelial cell apoptosis induced by micropatterned anisotropic geometry. Abstract Text:

Chia-Ching Wu,Yi-Shuan Li,Jason H Haga,Roland Kaunas,Jeng-Jiann Chiu,Fong-Chin Su,Shunichi Usami,Shu Chien,

To study the roles of anisotropic cell morphology and directionality of mechanical force in apoptosis, the spreading of human umbilical vein endothelial cells (HUVECs) was constrained by growing on micropatterned (MP) strips of fibronectin (FN, 20 microg/cm2) with widths of 15, 30, and 60 microm on silicone membrane. Cells on 30- and 60-microm strips, like cells on a nonpatterned (NP) surface coated with FN, showed clear actin stress fibers with anchoring spots of phosphorylated focal adhesion kinase (p-FAK) and no significant apoptosis. On 15-microm strips, cells had few stress fibers, no p-FAK, and significant apoptosis. After seeding for 12 h, the cells were subjected to pulsatile shear stress (12+/-4 dyn/cm2) parallel or perpendicular to MP strips, or kept under static condition. Parallel flow caused cell elongation with enhanced stress fibers and p-FAK, and a reduction in apoptosis, but perpendicular flow did not. The Rho inhibitory C3 exoenzyme abolished the effects of parallel flow. RhoV14, the constitutively active Rho, enhanced stress fibers and p-FAK, and prevented apoptosis of HUVECs on 15-microm strips under static condition. RhoV14 also reduced cell apoptosis under both parallel and perpendicular flows. Our results indicate that cell apoptosis can be modulated by changes in ECM micropatterning, anisotropic cell morphology, and mechanical forces. These extracellular microenvironment factors affect cell survival through alterations in Rho GTPase activity, stress fiber organization, and FAK phosphorylation.

Directional shear flow and Rho activation prevent the endothelial cell apoptosis induced by micropatterned anisotropic geometry. Publishing Authors By Initials

CC Wu,YS Li,JH Haga,R Kaunas,JJ Chiu,FC Su,S Usami,S Chien,

For similar enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: rho gtp-binding proteins research abstracts see: enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: rho gtp-binding proteins research

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Directional shear flow and Rho activation prevent the endothelial cell apoptosis induced by micropatterned anisotropic geometry. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 1254-9

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 17

MONTH: 01

YEAR: 2007

Directional shear flow and Rho activation prevent the endothelial cell apoptosis induced by micropatterned anisotropic geometry. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Directional shear flow and Rho activation prevent the endothelial cell apoptosis induced by micropatterned anisotropic geometry. Keywords Mesh Terms:

KEYWORDS: rho GTP-Binding Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Directional shear flow and Rho activation prevent the endothelial cell apoptosis induced by micropatterned anisotropic geometry. Information

Substance Name: rho GTP-Binding Proteins

Registry Number: EC 3.6.5.2

Grant and Affiliation Information for Directional shear flow and Rho activation prevent the endothelial cell apoptosis induced by micropatterned anisotropic geometry.

AFFILIATION: Department of Bioengineering and Whitaker Institute of Biomedical Engineering, University of California at San Diego, La Jolla, CA 92093, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL-085195

ACRONYM: HL

MEDLINETA: Proc Natl Acad Sci U S A

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