Directing RNA interference specifically to differentiated muscle cells.

Directing RNA interference specifically to differentiated muscle cells. Research Abstract Details 

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Directing RNA interference specifically to differentiated muscle cells. Abstract Text:

Carter A Herndon,Larry Fromm,

A common approach for mediating RNA interference (RNAi) is to introduce DNA that encodes short hairpin RNA (shRNA), which is often contained in a plasmid that can express a shRNA in a wide variety of cell types. Muscle cells and certain other cell types grown in culture can exist in both a dividing state and in a post-mitotic, differentiated state, and it is sometimes useful to induce RNAi selectively in terminally differentiated cells to study the function of a gene, particularly when the gene is also required for propagation of dividing cells. We describe two methods for studying gene function by RNAi specifically in terminally differentiated skeletal muscle cells in culture. We developed a shRNA expression vector, based on myosin light chain 1f gene regulatory sequences, which is designed to induce shRNA expression specifically after differentiation has been initiated. We show that this vector can mediate RNAi and is only active in differentiated muscle cells. Also, we developed an adenoviral vector that is designed to be able to deliver shRNAs directly to post-mitotic muscle cells. We show that adenoviruses produced using this vector mediate RNAi in differentiated muscle cells. These methods add to the repertoire of RNAi tools that can be used for identifying genes involved in any event of interest that occurs in differentiated muscle cells.

Directing RNA interference specifically to differentiated muscle cells. Publishing Authors By Initials

CA Herndon,L Fromm,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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MEDLINE DATE:

Directing RNA interference specifically to differentiated muscle cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of muscle research and cell motility

VOLUME: 28

Page Numbers: 11-7

Journal Abbreviation: J. Muscle Res. Cell. Motil.

ISSN: 0142-4319

DAY: 23

MONTH: 12

YEAR: 2006

Directing RNA interference specifically to differentiated muscle cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8006298

Directing RNA interference specifically to differentiated muscle cells. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: metabolism

Chemical & Substance for Abstract: Directing RNA interference specifically to differentiated muscle cells. Information

Substance Name: RNA, Small Interfering

Registry Number: 0

Grant and Affiliation Information for Directing RNA interference specifically to differentiated muscle cells.

AFFILIATION: Indiana University School of Medicine-Muncie, 2000 University Avenue, Muncie, IN 47306, USA.

Country: Netherlands

AGENCY: United States NINDS

GRANT: NS44924

ACRONYM: NS

MEDLINETA: J Muscle Res Cell Motil

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