Directing DNA methylation to inhibit gene expression.

Directing DNA methylation to inhibit gene expression. Research Abstract Details 

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Directing DNA methylation to inhibit gene expression. Abstract Text:

Andrew R Hoffman,Ji Fan Hu,Andrew R Hoffman,Ji Fan Hu,

: 1. DNA methylation is a critical epigenetic modification that silences gene transcription, participates in X-chromosome inactivation in females, and regulates genomic imprinting. 2. We have devised a method to inhibit transcriptional initiation by constructing short methylated oligonucleotides which induce DNA methylation at specific loci. 3. The methodology by which we devise these oligonucleotides is described, using oligonucleotides directed against the oncogene, Bcl-2.4. The human Bcl-2 gene contains two promoters, each of which contains a CpG island in its core region. Oligonucleotides are designed which can inhibit Bcl-2 transcription and lead to decreased mRNA and protein in vitro. When compared to standard anti-sense oligonucleotide action, these methylated oligonucleotides are far more sensitive and potentially, longer acting. 5. In principle, using this methodology, it should be possible to design methylated oligonucleotides that can methylate CpG islands and thereby downregulate any gene.

Directing DNA methylation to inhibit gene expression. Publishing Authors By Initials

AR Hoffman,JF Hu,AR Hoffman,JF Hu,

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Directing DNA methylation to inhibit gene expression. Journal Published:

PUBLICATION TYPE: Review

Journal: Cellular and molecular neurobiology

VOLUME: 26

Page Numbers: 425-38

Journal Abbreviation: Cell. Mol. Neurobiol.

ISSN: 0272-4340

DAY: 19

MONTH: 05

YEAR: 2006

Directing DNA methylation to inhibit gene expression. Information

Number of References: 44

LANGUAGE: eng

NlmUniqueID: 8200709

Directing DNA methylation to inhibit gene expression. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: genetics

Chemical & Substance for Abstract: Directing DNA methylation to inhibit gene expression. Information

Substance Name: bone growth factors

Registry Number: 0

Grant and Affiliation Information for Directing DNA methylation to inhibit gene expression.

AFFILIATION: Department of Medicine, Stanford University, Medical Service, VA Palo Alto Health Care System, Miranda Ave., Palo Alto, California 94304, USA. arhoffman@stanford.edu

Country: United States

AGENCY: United States NCI

GRANT: R43 CA86664-01

ACRONYM: CA

MEDLINETA: Cell Mol Neurobiol

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