Direct visualization of the EcoRII-DNA triple synaptic complex by atomic force microscopy.

Direct visualization of the EcoRII-DNA triple synaptic complex by atomic force microscopy. Research Abstract Details 

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Direct visualization of the EcoRII-DNA triple synaptic complex by atomic force microscopy. Abstract Text:

Luda S Shlyakhtenko,Jamie Gilmore,Alex Portillo,Gintautas Tamulaitis,Virginijus Siksnys,Yuri L Lyubchenko,Luda S Shlyakhtenko,Jamie Gilmore,Alex Portillo,Gintautas Tamulaitis,Virginijus Siksnys,Yuri L Lyubchenko,

Interactions between distantly separated DNA regions mediated by specialized proteins lead to the formation of synaptic protein-DNA complexes. This is a ubiquitous phenomenon which is critical in various genetic processes. Although such interactions typically occur between two sites, interactions among three specific DNA regions have been identified, and a corresponding model has been proposed. Atomic force microscopy was used to test this model for the EcoRII restriction enzyme and provide direct visualization and characterization of synaptic protein-DNA complexes involving three DNA binding sites. The complex appeared in the images as a two-loop structure, and the length measurements proved the site specificity of the protein in the complex. The protein volume measurements showed that an EcoRII dimer is the core of the three-site synaptosome. Other complexes were identified and analyzed. The protein volume data showed that the dimeric form of the protein is responsible for the formation of other types of synaptic complexes as well. The applications of these results to the mechanisms of the protein-DNA interactions are discussed.

Direct visualization of the EcoRII-DNA triple synaptic complex by atomic force microscopy. Publishing Authors By Initials

LS Shlyakhtenko,J Gilmore,A Portillo,G Tamulaitis,V Siksnys,YL Lyubchenko,LS Shlyakhtenko,J Gilmore,A Portillo,G Tamulaitis,V Siksnys,YL Lyubchenko,

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Direct visualization of the EcoRII-DNA triple synaptic complex by atomic force microscopy. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochemistry

VOLUME: 46

Page Numbers: 11128-36

Journal Abbreviation: Biochemistry

ISSN: 0006-2960

DAY: 11

MONTH: 09

YEAR: 2007

Direct visualization of the EcoRII-DNA triple synaptic complex by atomic force microscopy. Information

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LANGUAGE: eng

NlmUniqueID: 370623

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Grant and Affiliation Information for Direct visualization of the EcoRII-DNA triple synaptic complex by atomic force microscopy.

AFFILIATION: Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, 986025 Nebraska Medical Center, Omaha, Nebraska 68198-6025, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P20 RR16469

ACRONYM: RR

MEDLINETA: Biochemistry

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