Direct quantification of gene expression using capillary electrophoresis with laser-induced fluorescence.

Direct quantification of gene expression using capillary electrophoresis with laser-induced fluorescence. Research Abstract Details 

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Direct quantification of gene expression using capillary electrophoresis with laser-induced fluorescence. Abstract Text:

Jack G Goldsmith,Edidiong C Ntuen,Edie C Goldsmith,

Quantification of gene expression provides valuable information regarding the response of cells or tissue to stimuli and often is accomplished by monitoring the level of messenger RNA (mRNA) being transcribed for a particular protein. Although numerous methods are commonly used to monitor gene expression, including Northern blotting, real-time polymerase chain reaction, and RNase protection assay, each method has its own drawbacks and limitations. Capillary electrophoresis with laser-induced fluorescence (CE-LIF) can reduce protocol time, eliminate the need for radioactivity, and provide superior sensitivity and dynamic range for quantification of RNA. In addition, CE-LIF can be used to directly determine the amount of an RNA species present, something that is difficult and not normally accomplished using current methods. Gene expression is detected using a fluorescently labeled riboprobe specific for a given RNA species. This direct approach was validated by analyzing levels of 28S RNA and also used to determine the amount of discoidin domain receptor 2 mRNA in cardiac tissue.

Direct quantification of gene expression using capillary electrophoresis with laser-induced fluorescence. Publishing Authors By Initials

JG Goldsmith,EC Ntuen,EC Goldsmith,

For similar investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research abstracts see: investigative techniques: chemistry, analytical: photometry: luminescent measurements: fluorometry: spectrometry, fluorescence research

PUBMED ID PMID:

MEDLINE DATE:

Direct quantification of gene expression using capillary electrophoresis with laser-induced fluorescence. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical biochemistry

VOLUME: 360

Page Numbers: 23-9

Journal Abbreviation: Anal. Biochem.

ISSN: 0003-2697

DAY: 2

MONTH: 11

YEAR: 2006

Direct quantification of gene expression using capillary electrophoresis with laser-induced fluorescence. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370535

Direct quantification of gene expression using capillary electrophoresis with laser-induced fluorescence. Keywords Mesh Terms:

KEYWORDS: Spectrometry, Fluorescence

MESH TERMS: methods

Chemical & Substance for Abstract: Direct quantification of gene expression using capillary electrophoresis with laser-induced fluorescence. Information

Substance Name: RNA, Ribosomal, 28S

Registry Number: 0

Grant and Affiliation Information for Direct quantification of gene expression using capillary electrophoresis with laser-induced fluorescence.

AFFILIATION: Department of Chemistry, University of South Carolina-Aiken, Aiken, SC 29801, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL073937-03

ACRONYM: HL

MEDLINETA: Anal Biochem

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