Direct observation of regulated ribonucleoprotein transport across the nurse cell/oocyte boundary.

Direct observation of regulated ribonucleoprotein transport across the nurse cell/oocyte boundary. Research Abstract Details 

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Direct observation of regulated ribonucleoprotein transport across the nurse cell/oocyte boundary. Abstract Text:

Sarah Mische,Mingang Li,Madeline Serr,Thomas S Hays,

In Drosophila, the asymmetric localization of specific mRNAs to discrete regions within the developing oocyte determines the embryonic axes. The microtubule motors dynein and kinesin are required for the proper localization of the determinant ribonucleoprotein (RNP) complexes, but the mechanisms that account for RNP transport to and within the oocyte are not well understood. In this work, we focus on the transport of RNA complexes containing bicoid (bcd), an anterior determinant. We show in live egg chambers that, within the nurse cell compartment, dynein actively transports green fluorescent protein-tagged Exuperantia, a cofactor required for bcd RNP localization. Surprisingly, the loss of kinesin I activity elevates RNP motility in nurse cells, whereas disruption of dynein activity inhibits RNP transport. Once RNPs are transferred through the ring canal to the oocyte, they no longer display rapid, linear movements, but they are distributed by cytoplasmic streaming and gradually disassemble. By contrast, bcd mRNA injected into oocytes assembles de novo into RNP particles that exhibit rapid, dynein-dependent transport. We speculate that after delivery to the oocyte, RNP complexes may disassemble and be remodeled with appropriate accessory factors to ensure proper localization.

Direct observation of regulated ribonucleoprotein transport across the nurse cell/oocyte boundary. Publishing Authors By Initials

S Mische,M Li,M Serr,TS Hays,

For similar proteins: dna-binding proteins: trans-activators research abstracts see: proteins: dna-binding proteins: trans-activators research

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Direct observation of regulated ribonucleoprotein transport across the nurse cell/oocyte boundary. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular biology of the cell

VOLUME: 18

Page Numbers: 2254-63

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 11

MONTH: 04

YEAR: 2007

Direct observation of regulated ribonucleoprotein transport across the nurse cell/oocyte boundary. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201390

Direct observation of regulated ribonucleoprotein transport across the nurse cell/oocyte boundary. Keywords Mesh Terms:

KEYWORDS: Trans-Activators

MESH TERMS: metabolism

Chemical & Substance for Abstract: Direct observation of regulated ribonucleoprotein transport across the nurse cell/oocyte boundary. Information

Substance Name: Dynein ATPase

Registry Number: EC 3.6.4.2

Grant and Affiliation Information for Direct observation of regulated ribonucleoprotein transport across the nurse cell/oocyte boundary.

AFFILIATION: Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM53696

ACRONYM: GM

MEDLINETA: Mol Biol Cell

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