Direct monitoring of metal ion transfer between two trafficking proteins.

Direct monitoring of metal ion transfer between two trafficking proteins. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Direct monitoring of metal ion transfer between two trafficking proteins. Abstract Text:

Richard Ledwidge,Rebecca Soinski,Susan M Miller,

To avoid the toxic effects of both essential and nonessential metal ions, cells elaborate a variety of metal ion trafficking proteins that regulate metal ion mobility. While structures of several trafficking proteins have been determined, kinetic characterization of metal ion transfer between cognate protein partners and the factors controlling transfer has lagged behind, in part due to a limitation on methods to monitor the rapid transfer. In this Communication we report studies on the kinetics of Hg2+ transfer between separately expressed components of the flavoenzyme mercuric ion reductase (MerA) that take advantage of the sensitivity of the flavin fluorescence to the charge state of a cysteine thiol in the Hg2+ binding pathway. The thiolate form of C558 in the Tn501 MerA partially quenches the fluorescence of the oxidized enzyme. Protonation or binding of Hg2+ to the thiolate increases the fluorescence, providing a sensitive probe for kinetic analysis of the Hg2+ binding reaction. The kinetics of Hg2+ transfer in both directions between the cysteine pair of the separately expressed N-terminal domain and the C-terminal cysteines (C558, C559) of the catalytic core are presented, along with a model describing the overall process.

Direct monitoring of metal ion transfer between two trafficking proteins. Publishing Authors By Initials

R Ledwidge,R Soinski,SM Miller,

For similar proteins research abstracts see: proteins research

PUBMED ID PMID:

MEDLINE DATE:

Direct monitoring of metal ion transfer between two trafficking proteins. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 10842-3

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 10

MONTH: Aug

YEAR: 2005

Direct monitoring of metal ion transfer between two trafficking proteins. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Direct monitoring of metal ion transfer between two trafficking proteins. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Direct monitoring of metal ion transfer between two trafficking proteins. Information

Substance Name: mercuric reductase

Registry Number: EC 1.16.-

Grant and Affiliation Information for Direct monitoring of metal ion transfer between two trafficking proteins.

AFFILIATION: Department of Pharmaceutical Chemistry, 600 16th Street, University of California-San Francisco, San Francisco, CA 94143-2280, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Direct monitoring of metal ion transfer between two trafficking proteins Related Publications

• Bone morphogenetic protein-2 (BMP-2) and transforming growth factor-beta1 (TGF-beta1) alter connexin 43 phosphorylation in MC3T3-E1 Cells.
• Bovine abortion and neonatal death associated with Ureaplasma diversum.
• Characterization of growth and osteogenic differentiation of rabbit bone marrow stromal cells.
• Clinical evaluation of respiratory function in newborn infants.
• Conducting research: practical steps.
• Embryo transfer techniques in handling repeat breeding cows.
• Examining the effects of alcoholism typology and AA attendance on self-efficacy as a mechanism of change.
• Fibroblast growth factor receptor-1 interacts with the T-cell receptor signalling pathway.
• HIV testing among young adults in the United States: associations with financial resources and geography.
• Importance of diet of dam and colostrum to the biological antioxidant status and parenteral iron tolerance of the pig.
• Magnetic resonance imaging of microvascular leakage induced by myocardial contrast echocardiography in rats.
• Measurements of the anisotropy of ultrasonic attenuation in freshly excised myocardium.
• Measuring the quality of medication administration.
• Overexpression of BMP-2 modulates morphology, growth, and gene expression in osteoblastic cells.
• Personal perspectives: an interview with William R. Miller.
• Prediction of fertility of bovine semen: Preliminary studies with the hamster egg penetration test.
• Predictors of unsafe injecting drug use.
• Relationship of sialic acid and fatty acid composition of brain gangliosides: breast-fed vs formula-fed infant.
• Requirement for a localized, IP3R-generated Ca2+ transient during the furrow positioning process in zebrafish zygotes.
• Serial passage of MC3T3-E1 cells alters osteoblastic function and responsiveness to transforming growth factor-beta1 and bone morphogenetic protein-2.
• Spirituality: the silent dimension in addiction research. The 1990 leonard ball oration.
• Streamlined synthesis of phosphatidylinositol (PI), PI3P, PI3,5P2, and deoxygenated analogues as potential biological probes.
• Structure and measurement of dimensions of risk for HIV transmission in injecting drug users.
• Substrate-dependent dihydroxylation of substituted cyclopentenes: toward the syntheses of carbocyclic sinefungin and noraristeromycin.
• Survival of equine embryos co-cultured with equine oviductal epithelium from the four- to eight-cell to the blastocyst stage after transfer to synchronous recipient mares.
• The electrocardiogram in congenital malformations of the heart.
• Topical hyperbaric oxygen and low-energy laser for the treatment of chronic ulcers.
• Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development.
• Trials of vinorelbine and docetaxel in the treatment of advanced non small-cell lung cancer.
• Ultrastructural assessment of a blastocyst recovered from a cow with subclinical purulent endometritis.