Direct expression of a synthetic gene in Escherichia coli: purification and physicochemical properties of human initiation factor 4E.

Direct expression of a synthetic gene in Escherichia coli: purification and physicochemical properties of human initiation factor 4E. Research Abstract Details 

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Direct expression of a synthetic gene in Escherichia coli: purification and physicochemical properties of human initiation factor 4E. Abstract Text:

S Morino,M Yasui,M Doi,M Inoue,T Ishida,H Ueda,S Uesugi,

An artificial synthetic gene coding for human eIF-4E was cloned into an expression vector and direct expression was attempted in Escherichia coli [BL21(DE3) strain] under the control of T7 promoter. The active gene product which was induced in high yield (ca. 4 mg/100 ml) by isopropyl-beta-D-thiogalactopyranoside was purified to homogeneity by a two-step chromatographic procedure with a good yield (ca. 74%), and was confirmed to be recombinant human eIF-4E by amino acid composition and sequence analyses, isoelectric focusing, and absorption spectral measurements. The identity of three-dimensional structures between the recombinant and native human eIF-4Es was confirmed by CD and fluorescence measurements.

Direct expression of a synthetic gene in Escherichia coli: purification and physicochemical properties of human initiation factor 4E. Publishing Authors By Initials

S Morino,M Yasui,M Doi,M Inoue,T Ishida,H Ueda,S Uesugi,

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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Direct expression of a synthetic gene in Escherichia coli: purification and physicochemical properties of human initiation factor 4E. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 116

Page Numbers: 687-93

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1994

Direct expression of a synthetic gene in Escherichia coli: purification and physicochemical properties of human initiation factor 4E. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Direct expression of a synthetic gene in Escherichia coli: purification and physicochemical properties of human initiation factor 4E. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Direct expression of a synthetic gene in Escherichia coli: purification and physicochemical properties of human initiation factor 4E. Information

Substance Name: Recombinant Proteins

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Grant and Affiliation Information for Direct expression of a synthetic gene in Escherichia coli: purification and physicochemical properties of human initiation factor 4E.

AFFILIATION: Department of Physical Chemistry, Osaka University of Pharmaceutical Sciences.

Country: JAPAN

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MEDLINETA: J Biochem

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