Direct Demonstration of the Presence of Coordinated Sulfate in the Reaction Pathway of Arabidopsis thaliana Sulfite Oxidase Using (33)S Labeling and ESEEM Spectroscopy.

Direct Demonstration of the Presence of Coordinated Sulfate in the Reaction Pathway of Arabidopsis thaliana Sulfite Oxidase Using (33)S Labeling and ESEEM Spectroscopy. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Direct Demonstration of the Presence of Coordinated Sulfate in the Reaction Pathway of Arabidopsis thaliana Sulfite Oxidase Using (33)S Labeling and ESEEM Spectroscopy. Abstract Text:

Andrei V Astashkin,Kayunta Johnson-Winters,Eric L Klein,Robert S Byrne,Russ Hille,Arnold M Raitsimring,John H Enemark,Andrei V Astashkin,Kayunta Johnson-Winters,Eric L Klein,Robert S Byrne,Russ Hille,Arnold M Raitsimring,John H Enemark,Andrei V Astashkin,Kayunta Johnson-Winters,Eric L Klein,Robert S Byrne,Russ Hille,Arnold M Raitsimring,John H Enemark,

Sulfite oxidase from Arabidopsis thaliana has been reduced at pH = 6 with sulfite labeled with 33S (nuclear spin I = 3/2), followed by reoxidation by ferricyanide to generate the Mo(V) state of the active center. To obtain information about the hyperfine interaction (hfi) of 33S with Mo(V), continuous-wave electron paramagnetic resonance (EPR) and electron spin echo envelope modulation (ESEEM) experiments have been performed. The interpretation of the EPR and ESEEM spectra was facilitated by a theoretical analysis of the nuclear transition frequencies expected for the situation of the nuclear quadrupole interaction being much stronger than the Zeeman and hyperfine interactions. The isotropic hfi constant of 33S determined in these experiments was about 3 MHz, which demonstrates the presence of coordinated sulfate in the sulfite-reduced low-pH form of the plant enzyme.

Direct Demonstration of the Presence of Coordinated Sulfate in the Reaction Pathway of Arabidopsis thaliana Sulfite Oxidase Using (33)S Labeling and ESEEM Spectroscopy. Publishing Authors By Initials

AV Astashkin,K Johnson-Winters,EL Klein,RS Byrne,R Hille,AM Raitsimring,JH Enemark,AV Astashkin,K Johnson-Winters,EL Klein,RS Byrne,R Hille,AM Raitsimring,JH Enemark,AV Astashkin,K Johnson-Winters,EL Klein,RS Byrne,R Hille,AM Raitsimring,JH Enemark,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Direct Demonstration of the Presence of Coordinated Sulfate in the Reaction Pathway of Arabidopsis thaliana Sulfite Oxidase Using (33)S Labeling and ESEEM Spectroscopy. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of the American Chemical Society

VOLUME: 129

Page Numbers: 14800-10

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 6

MONTH: 11

YEAR: 2007

Direct Demonstration of the Presence of Coordinated Sulfate in the Reaction Pathway of Arabidopsis thaliana Sulfite Oxidase Using (33)S Labeling and ESEEM Spectroscopy. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Direct Demonstration of the Presence of Coordinated Sulfate in the Reaction Pathway of Arabidopsis thaliana Sulfite Oxidase Using (33)S Labeling and ESEEM Spectroscopy. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Direct Demonstration of the Presence of Coordinated Sulfate in the Reaction Pathway of Arabidopsis thaliana Sulfite Oxidase Using (33)S Labeling and ESEEM Spectroscopy. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Direct Demonstration of the Presence of Coordinated Sulfate in the Reaction Pathway of Arabidopsis thaliana Sulfite Oxidase Using (33)S Labeling and ESEEM Spectroscopy.

AFFILIATION: Contribution from the Department of Chemistry, University of Arizona, Tucson, Arizona 85721-0041, and Department of Biochemistry, University of California, Riverside, California 92521.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Direct Demonstration of the Presence of Coordinated Sulfate in the Reaction Pathway of Arabidopsis thaliana Sulfite Oxidase Using 33S Labeling and ESEEM Spectroscopy Related Publications

• 17O ESEEM evidence for exchange of the axial oxo ligand in the molybdenum center of the high pH form of sulfite oxidase.
• Direct Demonstration of the Presence of Coordinated Sulfate in the Reaction Pathway of Arabidopsis thaliana Sulfite Oxidase Using (33)S Labeling and ESEEM Spectroscopy.
• Effect of cervical anchorage studied by the implant method.
• Geometrical control of the active site electronic structure of pyranopterin enzymes by metal-dithiolate folding: aldehyde oxidase.
• Intramolecular electron transfer in a bacterial sulfite dehydrogenase.
• Photoelectron spectroscopy and electronic structure calculations of d1 vanadocene compounds with chelated dithiolate ligands: implications for pyranopterin Mo/W enzymes.
• Structural Studies of the Molybdenum Center of the Pathogenic R160Q Mutant of Human Sulfite Oxidase by Pulsed EPR Spectroscopy and (17)O and (33)S Labeling.
• Toward modeling the high chloride, low pH form of sulfite oxidase: K(a)-band ESEEM of equatorial chloro ligands in oxomolybdenum(V) complexes.