Direct and adaptor-mediated substrate recognition by an essential AAA+ protease.

Direct and adaptor-mediated substrate recognition by an essential AAA+ protease. Research Abstract Details 

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Direct and adaptor-mediated substrate recognition by an essential AAA+ protease. Abstract Text:

Peter Chien,Barrett S Perchuk,Michael T Laub,Robert T Sauer,Tania A Baker,Peter Chien,Barrett S Perchuk,Michael T Laub,Robert T Sauer,Tania A Baker,

Regulated proteolysis is required to execute many cellular programs. In Caulobacter crescentus, timely degradation of the master regulator CtrA by ClpXP protease is essential for cell-cycle progression and requires the colocalization of CtrA and RcdA. Here, we establish a biochemical framework to understand regulated proteolysis in C. crescentus and show that RcdA is not an adaptor for CtrA degradation. CtrA is rapidly degraded without RcdA and is recognized with an affinity comparable with the best ClpXP substrates. In contrast, SspBalpha, the alpha-proteobacterial homolog of SspB, functions as an adaptor to enhance degradation of specific substrates. Cargo-free SspBalpha is also itself a substrate of ClpXP-mediated proteolysis. Thus, our analysis (i) reveals the consequences of both direct and adaptor-stimulated recognition in mediating substrate specificity in vitro, (ii) reveals a potential regulatory role of controlled adaptor stability, and (iii) suggests that cell-cycle regulation of CtrA stability depends on repression of its intrinsic degradation rather than adaptor-mediated enhancement.

Direct and adaptor-mediated substrate recognition by an essential AAA+ protease. Publishing Authors By Initials

P Chien,BS Perchuk,MT Laub,RT Sauer,TA Baker,P Chien,BS Perchuk,MT Laub,RT Sauer,TA Baker,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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Direct and adaptor-mediated substrate recognition by an essential AAA+ protease. Journal Published:

PUBLICATION TYPE: Validation Studies

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 6590-5

Journal Abbreviation:

ISSN: 0027-8424

DAY: 9

MONTH: 04

YEAR: 2007

Direct and adaptor-mediated substrate recognition by an essential AAA+ protease. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Direct and adaptor-mediated substrate recognition by an essential AAA+ protease. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Direct and adaptor-mediated substrate recognition by an essential AAA+ protease. Information

Substance Name: Endopeptidase Clp

Registry Number: EC 3.4.21.92

Grant and Affiliation Information for Direct and adaptor-mediated substrate recognition by an essential AAA+ protease.

AFFILIATION: Department of Biology and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM-49224

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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