Diffusion-ordered nuclear magnetic resonance spectroscopy for analysis of DNA secondary structural elements.

Diffusion-ordered nuclear magnetic resonance spectroscopy for analysis of DNA secondary structural elements. Research Abstract Details 

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Diffusion-ordered nuclear magnetic resonance spectroscopy for analysis of DNA secondary structural elements. Abstract Text:

Attila Ambrus,Danzhou Yang,

Structure determination of secondary DNA structural elements, such as G-quadruplexes, gains an increasing importance as fundamental physiological roles are being associated with the formation of such structures in vivo. A truncated native DNA sequence generally requires further optimization to obtain a candidate with desired nuclear magnetic resonance (NMR) properties for structural analysis in solution. The optimum sequence is expected to form one dominant, stable molecular entity in solution with well-resolved NMR peaks. However, DNA sequences are prone to form structures composed of one, two, three, or four strands depending on sequence and solution conditions. The thorough characterization of the molecularity (stoichiometry and molecular weight) and appropriate solution conditions for sequences with different modifications traditionally applies analytical techniques that generally do not represent the solution conditions for NMR structure determination. Here we present the application of diffusion-ordered NMR spectroscopy as a useful analytical tool for the optimization and analysis of DNA secondary structural elements, specifically, the DNA G-quadruplex structures, including those formed in the human telomeric sequence and in the promoter regions of bcl-2 and c-myc genes.

Diffusion-ordered nuclear magnetic resonance spectroscopy for analysis of DNA secondary structural elements. Publishing Authors By Initials

A Ambrus,D Yang,

For similar chromosome structures: telomere research abstracts see: chromosome structures: telomere research

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Diffusion-ordered nuclear magnetic resonance spectroscopy for analysis of DNA secondary structural elements. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical biochemistry

VOLUME: 367

Page Numbers: 56-67

Journal Abbreviation: Anal. Biochem.

ISSN: 0003-2697

DAY: 25

MONTH: 04

YEAR: 2007

Diffusion-ordered nuclear magnetic resonance spectroscopy for analysis of DNA secondary structural elements. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370535

Diffusion-ordered nuclear magnetic resonance spectroscopy for analysis of DNA secondary structural elements. Keywords Mesh Terms:

KEYWORDS: Telomere

MESH TERMS: genetics

Chemical & Substance for Abstract: Diffusion-ordered nuclear magnetic resonance spectroscopy for analysis of DNA secondary structural elements. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Diffusion-ordered nuclear magnetic resonance spectroscopy for analysis of DNA secondary structural elements.

AFFILIATION: Department of Pharmacology and Toxicology, University of Arizona, Tucson, AZ 85721, USA. ambrus@pharmacy.arizona.edu

Country: United States

AGENCY: United States NCRR

GRANT: 1S10 RR16659

ACRONYM: RR

MEDLINETA: Anal Biochem

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