Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate.

Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate. Research Abstract Details 

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Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate. Abstract Text:

Mainak Das,Kerry Wilson,Peter Molnar,James J Hickman,Mainak Das,Kerry Wilson,Peter Molnar,James J Hickman,

This protocol describes a cell culture model to study the differentiation of fetal rat skeletal muscle cells. The model uses serum-free medium, a nonbiological substrate N-1[3(trimethoxysilyl)propyl] diethylenetriamine (DETA) and fabricated microcantilevers to promote the differentiation of dissociated rat myocytes into robust myotubes. In this protocol, we also describe how to characterize the myotubes on the basis of morphology, immunocytochemistry and electrophysiology. Here, four major techniques are employed: fabrication of cantilevers, surface modification of the glass and cantilever substrates with a DETA SAM, a serum-free medium and refined culture techniques. This culture system has potential applications in biocompatibility studies, bioartificial muscle engineering, skeletal muscle differentiation studies and for better understanding of myopathies and neuromuscular disorders. The model can be established in 26-33 d.

Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate. Publishing Authors By Initials

M Das,K Wilson,P Molnar,JJ Hickman,M Das,K Wilson,P Molnar,JJ Hickman,

For similar inorganic chemicals: elements: silicon research abstracts see: inorganic chemicals: elements: silicon research

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Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Nature protocols

VOLUME: 2

Page Numbers: 1795-801

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2007

Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate. Information

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LANGUAGE: eng

NlmUniqueID: 101284307

Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate. Keywords Mesh Terms:

KEYWORDS: Silicon

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate. Information

Substance Name: Silicon

Registry Number: 7440-21-3

Grant and Affiliation Information for Differentiation of skeletal muscle and integration of myotubes with silicon microstructures using serum-free medium and a synthetic silane substrate.

AFFILIATION: NanoScience Technology Center, University of Central Florida, Orlando, Florida 32826, USA.

Country: England

AGENCY: United States PHS

GRANT: 1 RO5 N5050452-03

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MEDLINETA: Nat Protoc

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