Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines.

Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines. Research Abstract Details 

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Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines. Abstract Text:

Alejandro ,Nalu Navarro-Alvarez,Debiao Zhao,Jorge D Rivas-Carrillo,Jane Lebkowski,Noriaki Tanaka,Ira J Fox,Naoya Kobayashi,Alejandro ,Nalu Navarro-Alvarez,Debiao Zhao,Jorge D Rivas-Carrillo,Jane Lebkowski,Noriaki Tanaka,Ira J Fox,Naoya Kobayashi,

This protocol describes a co-culture system for the in vitro differentiation of mouse embryonic stem cells into hepatocyte-like cells. Differentiation involves four steps: (i) formation of embryoid bodies (EB), (ii) induction of definitive endoderm from 2-d-old EBs, (iii) induction of hepatic progenitor cells and (iv) maturation into hepatocyte-like cells. Differentiation is completed by 16 d of culture. EBs are formed, and cells can be induced to differentiate into definitive endoderm by culture in Activin A and fibroblast growth factor 2 (FGF-2). Hepatic differentiation and maturation of cells is accomplished by withdrawal of Activin A and FGF-2 and by exposure to liver nonparenchymal cell-derived growth factors, a deleted variant of hepatocyte growth factor (dHGF) and dexamethasone. Approximately 70% of differentiated embryonic stem (ES) cells express albumin and can be recovered by albumin promoter-based cell sorting. The sorted cells produce albumin in culture and metabolize ammonia, lidocaine and diazepam at approximately two-thirds the rate of primary mouse hepatocytes.

Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines. Publishing Authors By Initials

A ,N Navarro-Alvarez,D Zhao,JD Rivas-Carrillo,J Lebkowski,N Tanaka,IJ Fox,N Kobayashi,A ,N Navarro-Alvarez,D Zhao,JD Rivas-Carrillo,J Lebkowski,N Tanaka,IJ Fox,N Kobayashi,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: mice research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: mice research

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Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature protocols

VOLUME: 2

Page Numbers: 347-56

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2007

Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101284307

Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines. Keywords Mesh Terms:

KEYWORDS: Mice

MESH TERMS: cytology

Chemical & Substance for Abstract: Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines. Information

Substance Name: Activins

Registry Number: 104625-48-1

Grant and Affiliation Information for Differentiation of mouse embryonic stem cells to hepatocyte-like cells by co-culture with human liver nonparenchymal cell lines.

AFFILIATION: Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.

Country: England

AGENCY: United States NIDDK

GRANT: DK48794

ACRONYM: DK

MEDLINETA: Nat Protoc

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