Differentiation of gastric surface mucous cells (GSM06) induced by air-liquid interface is regulated partly through mitogen-activated protein kinase pathway.

Differentiation of gastric surface mucous cells (GSM06) induced by air-liquid interface is regulated partly through mitogen-activated protein kinase pathway. Research Abstract Details 

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Differentiation of gastric surface mucous cells (GSM06) induced by air-liquid interface is regulated partly through mitogen-activated protein kinase pathway. Abstract Text:

Fumie Yokoyama,Yasuhisa Sakata,Akifumi Ootani,Takahiro Fujise,Takashi Kakimoto,Sadahiro Amemori,Ryosuke Shiraishi,Tsukasa Kuroki,Seiji Tsunada,Ryuichi Iwakiri,Kazuma Fujimoto,Fumie Yokoyama,Yasuhisa Sakata,Akifumi Ootani,Takahiro Fujise,Takashi Kakimoto,Sadahiro Amemori,Ryosuke Shiraishi,Tsukasa Kuroki,Seiji Tsunada,Ryuichi Iwakiri,Kazuma Fujimoto,Fumie Yokoyama,Yasuhisa Sakata,Akifumi Ootani,Takahiro Fujise,Takashi Kakimoto,Sadahiro Amemori,Ryosuke Shiraishi,Tsukasa Kuroki,Seiji Tsunada,Ryuichi Iwakiri,Kazuma Fujimoto,

Background and Aim: The aim of the present study was to examine the role of mitogen-activated protein (MAP) kinase pathway on gastric surface epithelium using an established cell culture model in which differentiation is promoted in GSM06 cells by air-liquid interface. Methods: A double-dish culture system of mouse gastric surface mucous cell line GSM06 in Ham's F12 medium supplemented with 10% fetal calf serum and 50 mug/mL gentamicin at 37 degrees C in a humidified atmosphere of 5% CO(2) in air was used for an air-liquid interface. Culture cells were examined on histology, cell proliferation was evaluated by bromodeoxy-uridine (BrdU) uptake, and western blot analysis of extracellular signal-regulated kinase (ERK)1/2 and phosphate ERK1/2. On day 3, U0126, an inhibitor of MAP kinase kinase (MEK), was added to medium of incubated cells. Results: GSM06 cells were differentiated with an air-liquid interface for 3 weeks. Compared to immersion control culture, phosphorylated ERK 1/2 expression increased significantly. This increase was completely suppressed with U0126, and tall columnar cells developed by air-liquid interface in GSM06 were not observed in U0126-treated cells. Increase in BrdU uptake with air-liquid interface was suppressed by U0126. Conclusion: These results suggested that MAP kinase signaling, activated by air-liquid interface, was, at least in part, related to cell differentiation in GSM06 cells induced by air-liquid interface.

Differentiation of gastric surface mucous cells (GSM06) induced by air-liquid interface is regulated partly through mitogen-activated protein kinase pathway. Publishing Authors By Initials

F Yokoyama,Y Sakata,A Ootani,T Fujise,T Kakimoto,S Amemori,R Shiraishi,T Kuroki,S Tsunada,R Iwakiri,K Fujimoto,F Yokoyama,Y Sakata,A Ootani,T Fujise,T Kakimoto,S Amemori,R Shiraishi,T Kuroki,S Tsunada,R Iwakiri,K Fujimoto,F Yokoyama,Y Sakata,A Ootani,T Fujise,T Kakimoto,S Amemori,R Shiraishi,T Kuroki,S Tsunada,R Iwakiri,K Fujimoto,

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Differentiation of gastric surface mucous cells (GSM06) induced by air-liquid interface is regulated partly through mitogen-activated protein kinase pathway. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of gastroenterology and hepatology

VOLUME: 22

Page Numbers: 2310-5

Journal Abbreviation: J. Gastroenterol. Hepatol.

ISSN: 0815-9319

DAY: 22

MONTH: Dec

YEAR: 2007

Differentiation of gastric surface mucous cells (GSM06) induced by air-liquid interface is regulated partly through mitogen-activated protein kinase pathway. Information

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LANGUAGE: eng

NlmUniqueID: 8607909

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Grant and Affiliation Information for Differentiation of gastric surface mucous cells (GSM06) induced by air-liquid interface is regulated partly through mitogen-activated protein kinase pathway.

AFFILIATION: Department of Internal Medicine and Gastrointestinal Endoscopy, Saga Medical School, Saga, Japan.

Country: Australia

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MEDLINETA: J Gastroenterol Hepatol

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