Differentiation of ES cells into cerebellar neurons.

Differentiation of ES cells into cerebellar neurons. Research Abstract Details 

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Differentiation of ES cells into cerebellar neurons. Abstract Text:

Enrique Salero,Mary E Hatten,

The neuronal circuits of the cerebellar cortex are essential for motor and sensory learning, associative memory formation, and the vestibular ocular reflex. In children and young adults, tumors of the granule cell, the medulloblastomas, represent 40% of brain tumors. We report the differentiation of E14 ES cells into mature granule neurons by sequential treatment with secreted factors (WNT1, FGF8, and RA) that initiate patterning in the cerebellar region of the neural tube, bone morphogenic proteins (BMP6/7 and GDF7) that induce early granule cell progenitor markers (MATH1, MEIS1, ZIC1), mitogens (SHH, JAG1) that control proliferation and induce additional granule cell markers (Cyclin D2, PAX2/6), and culture in glial-conditioned medium to induce markers of mature granule neurons (GABAalpha(6)r), including ZIC2, a unique marker for granule neurons. Differentiated ES cells formed classic "T-shaped" granule cell axons in vitro, and implantation of differentiated Pde1c-Egfp-BAC transgenic ES cells into the external granule cell layer of neonatal mice resulted in the extension of parallel fibers, migration across the molecular layer, incorporation into the internal granule cell layer, and extension of short dendrites, typical of young granule cells forming synaptic connections with afferent mossy fibers. These results underscore the utility of treating ES cells with local, inductive signals that regulate CNS neuronal development in vivo as a strategy for cell replacement therapy of defined neuronal populations.

Differentiation of ES cells into cerebellar neurons. Publishing Authors By Initials

E Salero,ME Hatten,

For similar nervous system: neurons research abstracts see: nervous system: neurons research

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Differentiation of ES cells into cerebellar neurons. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 2997-3002

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 9

MONTH: 02

YEAR: 2007

Differentiation of ES cells into cerebellar neurons. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Differentiation of ES cells into cerebellar neurons. Keywords Mesh Terms:

KEYWORDS: Neurons

MESH TERMS: cytology

Chemical & Substance for Abstract: Differentiation of ES cells into cerebellar neurons. Information

Substance Name: Culture Media, Conditioned

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Grant and Affiliation Information for Differentiation of ES cells into cerebellar neurons.

AFFILIATION: Laboratory of Developmental Neurobiology, The Rockefeller University, 1230 York Avenue, New York, NY 10021-6399, USA.

Country: United States

AGENCY: United States PHS

GRANT: R01 30532-04

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MEDLINETA: Proc Natl Acad Sci U S A

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