Differentiation between various effects of cytotoxic fractions of lymphocyte culture medium.

Differentiation between various effects of cytotoxic fractions of lymphocyte culture medium. Research Abstract Details 

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Differentiation between various effects of cytotoxic fractions of lymphocyte culture medium. Abstract Text:

B Wenzel,R Averdunk,

This paper describes the partial characterization of supernatants derived from mitogen stimulated lymphocytes by ammonium sulfate precipitation, ultrafiltration on Hollow-Fiber beakers and gelchromatography. Fractions from these purification procedures were tested on FL-target cells and the following target cell alterations were measured: RNA- and protein synthesis, 3H-TdR incorporation, cell number and lysis of target cells. Target cell alterations of control fractions from supernatants of non-stimulated lymphocytes, from culture medium and from FCS were compared to the effects of fractions from activated lymphocytes. 3H-TdR incorporation was not only inhibited by the fractions of activated lymphocyte cultures but also by fractions of various controls. Inhibition of 3H-TdR incorporation disappeared when control fractions were serially diluted. Only fractions of supernatant from activated lymphocytes could lyse target cells. It were the same fractions which still inhibited 3H-TdR incorporation of target cells after serial dilution and which showed stimulating effects on RNA synthesis. MW of fractions which were differentiated from control fractions with respect to target cell lysis were: 90,000, 45,000 and lower than 20,000 d.

Differentiation between various effects of cytotoxic fractions of lymphocyte culture medium. Publishing Authors By Initials

B Wenzel,R Averdunk,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research

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Differentiation between various effects of cytotoxic fractions of lymphocyte culture medium. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zeitschrift für Immunitätsforschung. Immunobiology

VOLUME: 153

Page Numbers: 380-94

Journal Abbreviation:

ISSN: 0340-904X

DAY: 12

MONTH: Dec

YEAR: 1977

Differentiation between various effects of cytotoxic fractions of lymphocyte culture medium. Information

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LANGUAGE: eng

NlmUniqueID: 7608602

Differentiation between various effects of cytotoxic fractions of lymphocyte culture medium. Keywords Mesh Terms:

KEYWORDS: RNA

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Differentiation between various effects of cytotoxic fractions of lymphocyte culture medium. Information

Substance Name: DNA

Registry Number: 9007-49-2

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Country: GERMANY, WEST

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MEDLINETA: Z Immunitatsforsch Immunobiol

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