Differentially expressed protein markers in human submandibular and sublingual secretions.

Differentially expressed protein markers in human submandibular and sublingual secretions. Research Abstract Details 

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Differentially expressed protein markers in human submandibular and sublingual secretions. Abstract Text:

Shen Hu,Patricia Denny,Paul Denny,Yongming Xie,Joseph A Loo,Lawrence E Wolinsky,Yang Li,Jim McBride,Rachel R Ogorzalek Loo,Mavash Navazesh,David T Wong,

Proteome analysis of secretions from individual salivary glands is important for understanding the health of the oral cavity and pathogenesis of certain diseases. However, cross-contamination of submandibular (SM) and sublingual (SL) glandular secretions can occur. The close anatomic relationship of the SM and SL ductal orifices can lead to such contamination. Additionally, these glands may share common ducts. To insure the purity of SM/SL secretions for proteomic analysis, it is important to develop unique biomarkers which could be used to verify the integrity of the individual glandular saliva. In this study, a proteomics approach based on mass spectrometry and gel electrophoresis techniques was utilized to identify and verify a set of proteins (cystatin C, calgranulin B and MUC5B mucin), which are differentially expressed in SM/SL secretions. SM/SL fluids were obtained from nine healthy subjects. Cystatin C was found to be an SM-selective protein as it was found in all SM fluids but not detected in two SL fluids. MUC5B mucin and calgranulin B, on the other hand, were found to be SL-selective proteins. All SL samples contained MUC5B mucin, whereas MUC5B mucin was not detected in four SM samples. Eight of the SL samples contained calgranulin B; however, calgranulin B was absent in eight SM samples. This set of protein markers, especially calgranulin B, can be used to determine the purity of SM/SL samples, and therefore identify potential individuals who do not exhibit cross-contaminated SM/SL secretions, an important requirement for subsequent proteome analysis of pure SM and SL secretions.

Differentially expressed protein markers in human submandibular and sublingual secretions. Publishing Authors By Initials

S Hu,P Denny,P Denny,Y Xie,JA Loo,LE Wolinsky,Y Li,J McBride,RR Ogorzalek Loo,M Navazesh,DT Wong,

For similar salivary glands: submandibular gland research abstracts see: salivary glands: submandibular gland research

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Differentially expressed protein markers in human submandibular and sublingual secretions. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: International journal of oncology

VOLUME: 25

Page Numbers: 1423-30

Journal Abbreviation: Int. J. Oncol.

ISSN: 1019-6439

DAY: 29

MONTH: Nov

YEAR: 2004

Differentially expressed protein markers in human submandibular and sublingual secretions. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9306042

Differentially expressed protein markers in human submandibular and sublingual secretions. Keywords Mesh Terms:

KEYWORDS: Submandibular Gland

MESH TERMS: secretion

Chemical & Substance for Abstract: Differentially expressed protein markers in human submandibular and sublingual secretions. Information

Substance Name: cystatin C

Registry Number: 0

Grant and Affiliation Information for Differentially expressed protein markers in human submandibular and sublingual secretions.

AFFILIATION: School of Dentistry, Dental Research Institute, UCLA School of Dentistry, University of California-Los Angeles, Los Angeles, CA 90095, USA.

Country: Greece

AGENCY: United States NIDCR

GRANT: U01 DE 15018

ACRONYM: DE

MEDLINETA: Int J Oncol

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