Differential regulation of Tbx5 protein expression and sub-cellular localization during heart development.

Differential regulation of Tbx5 protein expression and sub-cellular localization during heart development. Research Abstract Details 

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Differential regulation of Tbx5 protein expression and sub-cellular localization during heart development. Abstract Text:

Benjamin Bimber,Robert W Dettman,Hans-Georg Simon,

The T-box transcription factor Tbx5 can interact with Nkx2.5 and Gata4 transcription factors to synergistically regulate heart-specific genes in the nucleus. While a nuclear role for Tbx5 is clearly defined, we have previously shown that Tbx5 shuttles from nuclear to cytoplasmic sites, forming a complex with the PDZ-LIM protein LMP4 on the actin cytoskeleton. In this study, using a developmental series of chicken hearts, we provide the first evidence for differential Tbx5 protein expression and sub-cellular localization during cardiogenesis. At the tissue level, we show temporally and spatially restricted Tbx5 co-expression with LMP4. In cells co-expressing LMP4 and Tbx5 we demonstrate dynamic Tbx5 re-localization from exclusively nuclear to nuclear and cytoplasmic expression in the atrio-ventricular cushion. Furthermore, in coronary vessel development we show exclusive cytoplasmic localization of Tbx5, indicating a function for Tbx5 in the cytoplasm. In addition, we discover unknown regulation of Tbx5 and LMP4 expression in epicardial tissue, suggesting a specific role for Tbx5 in epicardial formation. These studies provide in vivo significance of the LMP4/Tbx5 protein interaction, suggesting both nuclear and cytoplasmic roles for Tbx5. The shuttling between nuclear and cytoplasmic sites reveals a novel mechanism for Tbx transcription factor regulation in chicken heart development allowing new insights for a better understanding of the molecular basis of hand/heart birth defects associated with TBX5 mutations.

Differential regulation of Tbx5 protein expression and sub-cellular localization during heart development. Publishing Authors By Initials

B Bimber,RW Dettman,HG Simon,

For similar proteins: dna-binding proteins: t-box domain proteins research abstracts see: proteins: dna-binding proteins: t-box domain proteins research

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Differential regulation of Tbx5 protein expression and sub-cellular localization during heart development. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Developmental biology

VOLUME: 302

Page Numbers: 230-42

Journal Abbreviation: Dev. Biol.

ISSN: 0012-1606

DAY: 16

MONTH: 09

YEAR: 2006

Differential regulation of Tbx5 protein expression and sub-cellular localization during heart development. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372762

Differential regulation of Tbx5 protein expression and sub-cellular localization during heart development. Keywords Mesh Terms:

KEYWORDS: T-Box Domain Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Differential regulation of Tbx5 protein expression and sub-cellular localization during heart development. Information

Substance Name: T-box transcription factor 5

Registry Number: 0

Grant and Affiliation Information for Differential regulation of Tbx5 protein expression and sub-cellular localization during heart development.

AFFILIATION: Department of Pediatrics, Northwestern University, The Feinberg School of Medicine, Children's Memorial Research Center, Chicago, IL 60614, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL085834-01

ACRONYM: HL

MEDLINETA: Dev Biol

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