Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish (Danio rerio).

Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish (Danio rerio). Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish (Danio rerio). Abstract Text:

Fu-Lung Yeh,Todd Hsu,

A spontaneous high expression of heat shock protein 70 (HSP 70) was found to arise in zebrafish (Danio rerio) at the larval stage (84 hr after fertilization). The level of HSP 70 in 84-hr-old larvae was estimated to be six- to eightfold that of 12-hr-old embryos. As heat-induced HSP 70 synthesis in many eukaryotic organisms is known to be mediated by a transcriptional-dependent pathway activated by heat shock factor 1 (HSF-1), we then examined if the spontaneous and heat-induced HSP 70 synthesis in zebrafish were controlled by the same mechanism. Although the transient increase of a 62-kDa HSF-1-like polypeptide in 72- to 96-hr-old larvae seemed to correlate with the onset of the spontaneous HSP 70 production, an anti-HSF-1 antibody cocktail supershifted the heat shock element (HSE) binding complex induced by stressed but not by unstressed zebrafish extracts. Northern blot and quantitative RT-PCR analysis demonstrated the predominant presence of the cognate form of hsp 70 mRNA (hsc 70 mRNA) in developing zebrafish. The extent of heat-induced HSP 70 production in 84-hr-old larvae matched well with a dramatic increase in hsp 70 mRNA accumulation, while no apparent increase in total hsp 70 mRNA could be detected in 72- to 84-hr-old unstressed larvae by northern blot analysis. The stable expression of hsc 70 mRNA specific to beta-actin mRNA in normal zebrafish was confirmed by RT-PCR analysis. Hence, the spontaneous high expression of HSP 70 in zebrafish is believed to be controlled by a mechanism different from the HSF-1-dependent transcriptional activation of hsp 70 under heat stress. J. Exp. Zool. 293:349-359, 2002.

Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish (Danio rerio). Publishing Authors By Initials

FL Yeh,T Hsu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish (Danio rerio). Journal Published:

PUBLICATION TYPE: Journal Article

Journal: The Journal of experimental zoology

VOLUME: 293

Page Numbers: 349-59

Journal Abbreviation: J. Exp. Zool.

ISSN: 0022-104X

DAY: 1

MONTH: Sep

YEAR: 2002

Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish (Danio rerio). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375365

Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish (Danio rerio). Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish (Danio rerio). Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish (Danio rerio).

AFFILIATION: Institute of Marine Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan, Republic of China.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Exp Zool

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish Danio rerio Related Publications

• A weighted zero-inflated Poisson model for estimation of recurrence of adenomas.
• An ACAP1-containing clathrin coat complex for endocytic recycling.
• Assessment of motion of a swing leg and gait rehabilitation with a gravity balancing exoskeleton.
• Asymmetric electrowetting--moving droplets by a square wave.
• Characterization of class 1 integrons and antimicrobial resistance in CTX-M-3-producing Serratia marcescens isolates from southern Taiwan.
• Differential regulation of spontaneous and heat-induced HSP 70 expression in developing zebrafish (Danio rerio).
• Diffusion-weighted magnetic resonance imaging of the liver in hepatitis B patients with Child-Pugh a cirrhosis.
• Discitis in an adult following acupuncture treatment: a case report.
• Effects of unidirectional permeability in asymmetric poly(DL-lactic acid-co-glycolic acid) conduits on peripheral nerve regeneration: an in vitro and in vivo study.
• Enamel diffusion modulated by Er:YAG laser (Part 1)--FRAP.
• Enamel diffusion modulated by Er:YAG laser (Part 2). Organic matrix.
• Erythropoiesis and myocardial energy requirements contribute to the hypermetabolism of childhood sickle cell anemia.
• Exploiting the TRIP-Br Family of Cell Cycle Regulatory Proteins as Chemotherapeutic Drug Targets in Human Cancer.
• Fabrication of highly rotational symmetric quasi-periodic structures by multiexposure of a three-beam interference technique.
• Family, peer and individual factors related to methylenedioxymethamphetamine use in Taiwanese adolescents.
• Linking the population epidemiology of acute renal failure, chronic kidney disease and end-stage renal disease.
• Nonunion of a sesamoid with tophaceous gout: a case report.
• Overexpression of KIAA0101 predicts high stage, early tumor recurrence, and poor prognosis of hepatocellular carcinoma.
• Pancoast syndrome caused by metastasis to the superior mediastinum of hepatocellular carcinoma.
• Performance study on newly developed impellers for a left ventricular assist device.
• Production of N-acetyl-D-neuraminic acid by recombinant whole cells expressing Anabaena sp. CH1 N-acetyl-D-glucosamine 2-epimerase and Escherichia coli N-acetyl-D-neuraminic acid lyase.
• Promoter region methylation and reduced expression of thrombospondin-1 after oxygen-glucose deprivation in murine cerebral endothelial cells.
• Radiofrequency ablation for hepatocellular carcinoma: a prospective comparison of four radiofrequency devices.
• Retrospective distortion correction for 3D MR diffusion tensor microscopy using mutual information and Fourier deformations.
• Roles of histamine receptors and oxyradicals in aggravation of acid-induced gastric haemorrhagic ulcers in endotoxaemic rats.
• Slow Acting Protein Extract from Fruit Pulp of Momordica charantia with Insulin Secretagogue and Insulinomimetic Activities.
• The Application of Non-Default Uncertainty Factors in the U.S. EPA's Integrated Risk Information System (IRIS). Part I: UF(L), UF(S), and "Other Uncertainty Factors".
• The dentate gyrus as a filter or gate: a look back and a look ahead.
• Tracheal incision for elective tracheotomy in oral cavity cancer.
• Unusually intense FDG uptake in a hypertrophied adenoid.