Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2.

Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2. Research Abstract Details 

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Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2. Abstract Text:

M H Vitaterna,C P Selby,T Todo,H Niwa,C Thompson,E M Fruechte,K Hitomi,R J Thresher,T Ishikawa,J Miyazaki,J S Takahashi,A Sancar,

Cryptochromes regulate the circadian clock in animals and plants. Humans and mice have two cryptochrome (Cry) genes. A previous study showed that mice lacking the Cry2 gene had reduced sensitivity to acute light induction of the circadian gene mPer1 in the suprachiasmatic nucleus (SCN) and had an intrinsic period 1 hr longer than normal. In this study, Cry1(-/-) and Cry1(-/-)Cry2(-/-) mice were generated and their circadian clocks were analyzed at behavioral and molecular levels. Behaviorally, the Cry1(-/-) mice had a circadian period 1 hr shorter than wild type and the Cry1(-/-)Cry2(-/-) mice were arrhythmic in constant darkness (DD). Biochemically, acute light induction of mPer1 mRNA in the SCN was blunted in Cry1(-/-) and abolished in Cry1(-/-)Cry2(-/-) mice. In contrast, the acute light induction of mPer2 in the SCN was intact in Cry1(-/-) and Cry1(-/-)Cry2(-/-) animals. Importantly, in double mutants, mPer1 expression was constitutively elevated and no rhythmicity was detected in either 12-hr light/12-hr dark or DD, whereas mPer2 expression appeared rhythmic in 12-hr light/12-hr dark, but nonrhythmic in DD with intermediate levels. These results demonstrate that Cry1 and Cry2 are required for the normal expression of circadian behavioral rhythms, as well as circadian rhythms of mPer1 and mPer2 in the SCN. The differential regulation of mPer1 and mPer2 by light in Cry double mutants reveals a surprising complexity in the role of cryptochromes in mammals.

Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2. Publishing Authors By Initials

MH Vitaterna,CP Selby,T Todo,H Niwa,C Thompson,EM Fruechte,K Hitomi,RJ Thresher,T Ishikawa,J Miyazaki,JS Takahashi,A Sancar,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 96

Page Numbers: 12114-9

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 12

MONTH: Oct

YEAR: 1999

Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: genetics

Chemical & Substance for Abstract: Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2. Information

Substance Name: cryptochrome protein, Drosophila

Registry Number: 0

Grant and Affiliation Information for Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2.

AFFILIATION: Department of Neurobiology, Northwestern University, Evanston, IL 60208, USA.

Country: UNITED STATES

AGENCY: United States NIGMS

GRANT: GM31082

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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