Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates.

Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates. Research Abstract Details 

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Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates. Abstract Text:

K B Adler,Y Li,

The effects of extracellular nucleotide triphosphates on the stimulation of mucin production by airway epithelial cells were examined. The order of potency in stimulating mucin secretion in primary cultures of human tracheobronchial epithelial cells is: uridine 5'-triphosphate (UTP) approximately equal to adenosine 5'-triphosphate (ATP) approximately equal to ATP-gamma-S > uridine 5'-diphosphate approximately equal to adenosine 5'-diphosphate > alpha,beta-methylene ATP >> adenosine. However, only UTP can increase mucin gene (MUC5AC, MUC5B) expression; ATP and other analogues have no stimulatory effect. The stimulation of MUC5AC and MUC5B expression by UTP is time- and dose-dependent. A similar effect on the elevation of mucous cell population in mouse airway epithelium can be demonstrated in vivo by an intratracheal instillation of UTP-saline solution. The stimulatory effect of UTP or ATP on mucin secretion was inhibited by pertussis toxin, U73122, and Calphostin C, but not by PD98059, suggesting a G-protein/ phospholipase (PL) C/protein kinase (PK) C-dependent and mitogen-activated protein kinase (MAPK)-independent signaling pathway. However, the stimulatory effect of UTP on mucin gene expression was sensitive to pertussis toxin and PD98059, but not to Calphostin C and U73122, suggesting a G-protein/MAPK-dependent and PLC/PKC-independent signaling pathway. These findings are the first demonstration that UTP, a pyrimidine nucleotide triphosphate, can enhance both mucin secretion and mucin gene expression through different signaling pathways.

Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates. Publishing Authors By Initials

KB Adler,Y Li,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: pyrimidines: pyrimidine nucleotides: uracil nucleotides: uridine triphosphate research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: pyrimidines: pyrimidine nucleotides: uracil nucleotides: uridine triphosphate research

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Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: American journal of respiratory cell and molecular

VOLUME: 25

Page Numbers: 409-17

Journal Abbreviation: Am. J. Respir. Cell Mol. Biol.

ISSN: 1044-1549

DAY: 29

MONTH: Oct

YEAR: 2001

Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8917225

Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates. Keywords Mesh Terms:

KEYWORDS: Uridine Triphosphate

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates. Information

Substance Name: Protein Kinase C

Registry Number: EC 2.7.11.13

Grant and Affiliation Information for Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates.

AFFILIATION: Center for Comparative Respiratory Biology and Medicine, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL 35635

ACRONYM: HL

MEDLINETA: Am J Respir Cell Mol Biol

REFSOURCE: Am J Respir Cell Mol Biol. 2001 Oct;25(4

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