Differential NOS expression in freshwater and aestivating Protopterus dolloi (lungfish): Heart vs kidney readjustments.

Differential NOS expression in freshwater and aestivating Protopterus dolloi (lungfish): Heart vs kidney readjustments. Research Abstract Details 

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Differential NOS expression in freshwater and aestivating Protopterus dolloi (lungfish): Heart vs kidney readjustments. Abstract Text:

Daniela Amelio,Filippo Garofalo,Elvira Brunelli,Ai May Loong,Wai Peng Wong,Yuen Kwong Ip,Bruno Tota,Maria Carmela Cerra,Daniela Amelio,Filippo Garofalo,Elvira Brunelli,Ai May Loong,Wai Peng Wong,Yuen Kwong Ip,Bruno Tota,Maria Carmela Cerra,

African lungfish Protopterus dolloi is an obligatory air-breather, which aestivates in a cocoon during the dry season. Aestivation associates with functional modifications in many tissues and organs, including heart and kidney. Due to its pleiotropic modulatory effects, nitric oxide (NO), generated by nitric oxide synthases (NOSs), may coordinate organ rearrangement, allowing adaptive adjustments under stressful environmental conditions. By immunofluorescence, Western blotting and NADPH-diaphorase, we examined cardiac and renal localization and activity of NOSs isoforms in both freshwater (FW) and aestivating [6 days (6DA) and 40 days (40DA) of estivation] P. dolloi. In heart and kidney endothelial NOS (eNOS) is the major isoform with respect to inducible and neuronal NOS (iNOS and nNOS, respectively). Cardiac eNOS locates in the epicardium, the trabecular endothelial endocardium, and myocardiocytes of both FW and aestivating fish. Western blotting revealed that cardiac eNOS expression increases in 6DA, but decreases in 40DA fish. In FW fish kidney eNOS is present in vascular endothelial cells and in podocytes of renal corpuscles. In tubular epithelial cells it is restricted to the apical pole. With aestivation, both renal localization and expression of eNOS increase. NADPH-diaphorase revealed an enhancement of cardiac and renal NOS activities during aestivation. Results suggest that in P. dolloi NO contributes, in an autocrine-paracrine fashion, to cardiac and renal readjustments during aestivation. Our findings are of evolutionary interest, since they document for the first time the presence of a NOS system in a ancestral fish, indicative of deep phylogenetic roots of NO bio-synthesis.

Differential NOS expression in freshwater and aestivating Protopterus dolloi (lungfish): Heart vs kidney readjustments. Publishing Authors By Initials

D Amelio,F Garofalo,E Brunelli,AM Loong,WP Wong,YK Ip,B Tota,MC Cerra,D Amelio,F Garofalo,E Brunelli,AM Loong,WP Wong,YK Ip,B Tota,MC Cerra,

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Differential NOS expression in freshwater and aestivating Protopterus dolloi (lungfish): Heart vs kidney readjustments. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nitric oxide : biology and chemistry / official jo

VOLUME: 18

Page Numbers: 1-10

Journal Abbreviation: Nitric Oxide

ISSN: 1089-8603

DAY: 30

MONTH: 10

YEAR: 2007

Differential NOS expression in freshwater and aestivating Protopterus dolloi (lungfish): Heart vs kidney readjustments. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Differential NOS expression in freshwater and aestivating Protopterus dolloi (lungfish): Heart vs kidney readjustments.

AFFILIATION: Department of Cellular Biology, University of Calabria, 87030 Arcavacata di Rende, CS, Italy; Department of Pharmaco-Biology, University of Calabria, 87030 Arcavacata di Rende, CS, Italy.

Country: United States

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MEDLINETA: Nitric Oxide

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